Spared Nerve Injury Model
Spared Nerve Injury Model - Related Videos
07:44
The Spared Nerve Injury (SNI) Model of Induced Mechanical Allodynia in Mice
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Cited by 97 •
2011
The Spared Nerve Injury animal model is described here as a mouse model of peripheral neuropathic pain following partial denervation of the sciatic nerve by lesioning the tibial and common peroneal nerve branches, leaving the remaining sural nerve intact. Behavioral modification resulting from mechanical allodynia is quantified by von Frey filaments.
03:34
Inducing a Facial Nerve Injury in a Rat Model
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2025
This video demonstrates a surgical protocol on a rat model, detailing steps from anesthesia preparation to facial nerve injury induction. This protocol is useful for studying nerve regeneration and surgical techniques in medical research.
04:34
Modified Spared Nerve Injury Surgery Model of Neuropathic Pain in Mice
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Cited by 5 •
2022
The modified surgery is a simplified method for mouse or rat spared nerve injury model that requires only one ligation and one cut to injure both common peroneal and sural nerves.
03:13
Construction of a Composite Regenerative Peripheral Nerve Interface in a Rat Model of Nerve Injury
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2025
Source: Svientek, S. R., et al. Fabrication of the Composite Regenerative Peripheral Nerve Interface (C-RPNI) in the Adult Rat. J. Vis. Exp. (2020).This video demonstrates the surgical construction of a composite regenerative peripheral nerve interface (C-RPNI) in a rat model of peripheral nerve injury. The procedure involves attaching a transected peroneal nerve to a muscle graft and overlaying it with a dermal skin graft to promote nerve regeneration. Over time, motor and sensory neurons...
07:42
Nerve-sparing Mid-urethral Obstruction (NeMO) in Female Small Rodents
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Cited by 7 •
2017
Traditional modeling of partial bladder outlet obstruction in rodents is fraught with animal mortality. A denervation injury from dissection around the proximal urethra and bladder neck is also of major concern. We developed and evaluated a safe and reliable mid-urethral obstruction model, avoiding the shortcomings of the traditional model.
FAQs
Preserving the sural nerve creates a defined contrast between injured and intact peripheral nerve territories within the same limb. This arrangement allows researchers to examine how damage to selected sciatic branches affects sensory signaling beyond the directly injured nerves. The intact branch therefore helps reveal persistent sensory abnormalities and altered pain processing associated with neighboring nerve injury.
The model links peripheral nerve damage with changes in pain signaling at more central levels. Persistent sensory abnormalities provide a behavioral indication that signaling has been altered, while biological studies can examine neuroimmune interactions and central sensitization, meaning heightened responsiveness within pain-processing pathways. Together, these features support investigation of how local nerve injury contributes to ongoing neuropathic pain.
Mechanical allodynia and hyperalgesia are key sensory outcomes associated with the model. Allodynia refers to pain-related responses to normally nonpainful mechanical stimulation, whereas hyperalgesia describes an increased response to painful stimulation. Tracking these abnormalities gives researchers behavioral evidence of altered pain signaling and helps compare disease progression or responses to experimental interventions.
Establishment typically requires identifying selected branches of the sciatic nerve and then ligating or transecting the tibial and common peroneal branches while leaving the sural nerve intact. The resulting nerve arrangement produces persistent sensory abnormalities in the affected limb. Researchers can then assess behavioral and biological outcomes to study neuropathic pain mechanisms.
Researchers use the model when they need a reproducible experimental setting for testing analgesic drugs or other interventions against neuropathic pain-related abnormalities. Mechanical allodynia, hyperalgesia, and altered pain signaling provide measurable outcomes for comparing treatment effects. These results can support therapeutic development by showing whether an intervention changes established sensory or biological consequences of nerve damage.
In medicine, the model supports research into the consequences of peripheral nerve damage, including how injury produces persistent pain and changes sensory processing. Its reproducible behavioral and biological outcomes make it useful for connecting pain mechanisms with candidate therapies. Studies can therefore examine both disease-related processes and interventions intended to reduce neuropathic pain.