3d Bioprinting
3d Bioprinting - Related Videos
03:13
A Method for 3D Bioprinting Murine Cortical Astrocytes
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2025
The video demonstrates the process of creating a 3D bioprinted construct using astrocytes suspended in a bioink solution. The bioprinted construct, after crosslinking, facilitates astrocyte adhesion and their spreading, forming neural-like tissue.
04:40
Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting
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Cited by 55 •
2017
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
08:31
Bioprinting of Hydrogel Tumor Slices as a 3D Model for Mantle Cell Lymphoma
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2025
This protocol provides a detailed description of the generation, culture, and analysis of mantle cell lymphoma in 3D printed hydrogel tumor slices.
04:41
Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts
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2023
This video explains the 3D magnetic bioprinting of co-cultured pancreatic cancer cells and fibroblasts. Pancreatic cancer cells and activated pancreatic fibroblast cells are co-cultured and magnetized using a biocompatible nanoparticle assembly, to generate spheroids, under the influence of magnetic forces.
08:34
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
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Cited by 59 •
2016
We describe a set of protocols that together provide a tissue-mimicking hydrogel bioink with which functional and viable 3-D tissue constructs can be bioprinted for use in in vitro screening applications.
FAQs
Its composition is central because bioinks may contain living cells, hydrogels, or other biomaterials that provide the immediate environment for the construct. The printed material must also be stabilized under conditions that support cell survival and maturation. These features influence whether the resulting structure can maintain its intended organization and remain useful for modeling neural tissue.
A digital design specifies where deposited material and cells are positioned during layer-by-layer construction. This provides greater control over cellular placement than an unstructured arrangement and helps reproduce selected aspects of tissue architecture. In neural models, that control can be used to organize multicellular constructs or brain-like scaffolds for studying how tissue structure relates to biological behavior.
Deposition alone does not ensure that a construct will remain suitable for biological study. Stabilization helps preserve the printed arrangement while maintaining conditions that support cell survival and maturation. As cells develop within the stabilized structure, the construct can better represent aspects of neural tissue organization, making it more relevant for developmental, disease, or drug-response research.
A typical workflow begins with a digital design that represents the intended structure. Bioink containing cells, hydrogels, or other biomaterials is then deposited layer by layer according to that design. The resulting construct is stabilized under conditions that support cell survival and maturation. This sequence links planned architecture with a living, organized model for subsequent study.
These constructs can support investigations of neural development, disease, and responses to drugs. Neural tissue models, brain-like scaffolds, and multicellular structures each provide ways to reproduce selected features of tissue organization while controlling cellular placement and the surrounding extracellular environment. Their value lies in extending in vitro research beyond simpler arrangements that provide less architectural control.
By controlling cellular placement and the extracellular environment, 3D bioprinting can produce organized neural constructs that may inform research on tissue restoration. Brain-like scaffolds and multicellular models can help examine how neural cells behave within designed structures. Although the overview presents neural repair as a future direction, the immediate relevance is improved experimental modeling of neural tissue organization.