Hek293t Cells
Hek293t Cells - Related Videos
06:39
High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
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Cited by 3 •
2022
In this protocol, quantum dots conjugated to recombinant SARS-CoV-2 spike enable cell-based assays to monitor spike binding to hACE2 at the plasma membrane and subsequent endocytosis of the bound proteins into the cytoplasm.
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Cited by 7 •
2017
We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.
03:11
Polyethylenimine-Mediated Transfection of Embryonic Kidney Cells for Recombinant Virus Production
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2026
Source: Van Lidth de Jeude, et al. A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids. J. Vis. Exp. (2015).This video demonstrates the process of producing viral particles through transfection of embryonic kidney cells with plasmid–polyethylenimine complexes, which leads to viral genome expression, particle assembly, and virus collection in the medium.
04:16
Cell Aggregation Assay to Detect Trans Interactions between Cell Adhesion Molecules
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2025
In this video, we demonstrate the technique to measure transcellular interactions between synaptic cell adhesion molecules through the HEK cell aggregation assay. In this assay, an equal number of HEK cells expressing a desired presynaptic cell adhesion molecule and a fluorescent protein are mixed with HEK cells expressing a post-synaptic cell adhesion molecule and another fluorescent protein. The interaction between the cell adhesion molecules forces the cells to aggregate, which can be...
11:21
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
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Cited by 3 •
2017
The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.
FAQs
The SV40 large T antigen supports replication of plasmids that contain an SV40 origin. This allows those plasmids to remain active in the cell system and contributes to efficient transient transfection. In practice, the mechanism helps researchers obtain strong expression from introduced constructs, which is useful when testing neuronal proteins or generating recombinant products.
Their efficient transient transfection makes it practical to introduce expression plasmids and obtain substantial production of the encoded protein. The SV40 large T antigen further supports plasmids carrying the matching origin, reinforcing plasmid-based expression. This combination makes the cells useful when researchers need abundant recombinant protein for molecular biology or neuroscience experiments.
Researchers can examine neuronal receptors, ion channels, signaling proteins, and reporter constructs under controlled conditions. Testing these components separately helps clarify their behavior without beginning immediately in neurons, where many cellular processes operate together. Results from the cell-based system can guide subsequent studies of neuronal mechanisms and gene function.
A study generally begins by selecting a construct encoding a receptor, ion channel, signaling protein, or reporter. Researchers then use transient transfection to introduce it into the cells and test the resulting expression or activity in a controlled setting. This workflow supports an initial evaluation before the construct is examined in neurons or used in a broader experiment.
HEK293T cells provide a cellular platform for producing recombinant viral vectors. In neuroscience, these vectors can support experiments that manipulate gene function or neural circuits. Using the cells at this production stage allows investigators to develop and evaluate tools before applying them to studies involving neuronal systems, where the vectors may be used for targeted biological manipulation.
The cell line offers a controlled preliminary system for evaluating receptors, channels, signaling proteins, and reporter constructs. Researchers can first assess whether a gene or molecular tool produces the intended expression or experimental signal, then move to neuronal studies with more focused questions. This staged approach connects molecular testing with investigations of neural function and circuit manipulation.