Exogenous Ketones
Exogenous Ketones - Related Videos
03:09
Identification of Unknown Aldehydes and Ketones - Concepts
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2020
Aldehydes and Ketones Aldehydes and ketones have a carbonyl group (C=O) as a functional group. A ketone has two alkyl or aryl groups attached to the carbonyl carbon (RCOR’). The simplest ketone is acetone, which has two methyl groups attached to the carbonyl carbon (CH3COCH3). An aldehyde is similar to a ketone, except that instead of two side groups connected to the carbonyl carbon, they have at least one hydrogen (RCOH). The simplest aldehyde is formaldehyde (HCOH), as it has two hydrogens...
15:03
Identification of Unknown Aldehydes and Ketones - Student Protocol
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2020
Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA DNPH Test for Aromatic Aldehydes and KetonesExpand In this lab, you'll use the DNPH test, the Tollens' test, and the iodoform test to identify two unknown aldehydes or ketones. You'll use butanone and benzaldehyde as known compounds to confirm that the tests are working as expected. You'll start the lab with the DNPH test, which relies on the reaction between 2,4-dinitrophenylhydrazine, or DNPH,...
05:35
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Cited by 4 •
2016
Here we report protocols to detect endogenous and exogenous centromere-kinetochore proteins in human cells and quantify these protein levels at centromeres-kinetochores by indirect immunofluorescent staining through the use of fixation (paraformaldehyde, acetone, or methanol fixation).
02:40
Inactivation of Bacteriophages Using Exogenous Lipopolysaccharide to Generate Phage-Free Salmonella Cultures
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2026
Source: Fernández-Fernández, R., et. al. Bacteriophage Removal from Infected Salmonella Cultures. J. Vis. Exp. (2024)This video demonstrates the use of exogenous LPS (Lipopolysaccharide) as a receptor mimic to inactivate lytic bacteriophages in Salmonella cultures. Through sequential incubation, centrifugation, and washing, phages are eliminated, resulting in a viable, phage-free bacterial suspension for downstream applications.
05:30
Exogenous Mitochondrial Transfer in Differentiating Brown Adipocytes and AGPAT2-Deficient Preadipocytes
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2026
Exogenous mitochondria of human and murine origin can be internalized by differentiating preadipocytes and tracked throughout adipogenic differentiation using imaging and genetic tools. While these mitochondria physically interact with the endogenous mitochondrial network, they do not increase the expression of markers associated with mature brown adipocytes.
FAQs
After entering the bloodstream, ketone bodies are taken up by tissues and converted to acetyl-CoA. Mitochondria can then use this molecule for energy production, allowing investigators to examine ketone utilization without requiring fasting or carbohydrate restriction. This pathway helps connect circulating ketone concentrations with tissue-level changes in energy metabolism.
Formulation and administration route influence how quickly and effectively circulating β-hydroxybutyrate and acetoacetate rise. Oral and intravenous delivery therefore provide different pharmacological conditions for studying ketone exposure. Comparing these approaches can help researchers distinguish effects associated with delivery method from effects associated with the ketone compounds themselves.
The recipient’s metabolic state can modify responses to externally supplied ketones, including effects on glucose and insulin metabolism. Dose and formulation also contribute to this variability. Accounting for these factors is important when interpreting changes in circulating ketones or energy use, because the same intervention may produce different outcomes in different metabolic conditions.
Exogenous ketones provide experimental tools for examining both ketone-body signaling and energy metabolism. Because administration can raise circulating β-hydroxybutyrate and acetoacetate independently of fasting or carbohydrate restriction, researchers can study ketone-related effects under controlled conditions. The approach also supports investigation of how ketone exposure relates to metabolic flexibility, the capacity to adjust energy use.
A study should specify the ketone formulation, dose, and route of administration, then consider the participant’s or experimental subject’s metabolic state. Researchers can relate these conditions to circulating β-hydroxybutyrate and acetoacetate and to changes in glucose or insulin metabolism. This framework helps separate exposure-related effects from broader nutritional or metabolic influences.
These compounds are being studied because they connect circulating ketone availability with mitochondrial energy production, ketone-body signaling, and glucose and insulin metabolism. Those mechanisms are relevant across neurological, metabolic, and cardiovascular conditions. In pharmacology, exogenous ketones therefore serve both as potential investigational interventions and as tools for examining how altered energy metabolism affects disease-related processes.