Insect Puparium verwijdering: een methode voor poponderzoek

-To gain access to the developing pupa, remove the puparium after the pupal epithelium has separated from the cuticle or after pupal stage P5. To begin, place the intact pupa with its ventral side facing towards you. Use forceps to hold the puparium at the empty space between the anterior end of the casing and the pupa's head. Then, gently break and remove the anterior piece of the puparium to expose the head.

While keeping the ventral orientation, use the forceps to create small breaks along the anterior-posterior axis of the puparium and ensure a continuous gap is formed in the process. Be careful not to damage any of the pupa tissue directly underneath. Then continue to break the puparium from the gap outward, one portion at a time, and spread out the torn casing in the process. Using a fine wet paintbrush, pick up the exposed pupa for further investigation. In the example protocol, we will see how to remove the puparium of Drosophila guttifera and prepare the pupa for imaging.

-To begin puparium removal, affix a piece of double-sided tape on a piece of paper towel. Place the pupa on the double-sided tape, ventral side up. Locate the space between the anterior side of the pupari