Levensduurprotocol voor Drosophila melanogaster:overlevingscurven genereren om verschillen in vlieglevensduur te identificeren

- Start by cohousing a set number of male and female Drosophila melanogaster fruit flies in a vial containing cornmeal medium. This allows for a consistent density of offspring.

Allow the female flies to lay eggs. Then remove the adult flies. The eggs will become larvae, pupae, and finally adult flies in a span of 10 days. New flies emerging earlier than their cohort should be removed before day 9. Divide the now synchronized flies into groups for the control and experimental conditions. Cohousing male and female flies allows mating, removing a variable that affects lifespan.

After several days, separate the flies by sex into groups. Place an equal number of flies into the corresponding experimental condition. Every three days, transfer the living flies into a new vial and record the number of dead flies. Repeat this process until all the flies have died.

After at least three replicate experiments, plot the mean percentage of surviving flies by day to generate mean survival curves for each condition. In this example, we will see how to generate survival curves for cohorts treated with endocrine-disrupting chemicals.

- In the lifespan protocol, first set up 20 vials with eight femal