Injectie van volwassen Drosophila-vliegen: een methode voor samengestelde of labelafgifte

- Prepare tapered injection needles by pulling thin-walled glass capillaries using a needle puller. Hold a needle under a microscope and break the tip, making sure that the opening is just large enough to pierce the adult fly cuticle without wounding it more than necessary. Measure the injection volume by loading your needle with a sterile dye and salt buffer solution and then ejecting it onto a drop of mineral oil on a stage micrometer. Use the scale on the micrometer to measure the diameter of the ejected droplet, which can be used to calculate the volume dispensed, and calibrate your injector settings accordingly.

Load the needle with your injection solution and mount it onto an injection nozzle. Anesthetize your flies with CO2 and arrange them on a CO2 fly pad that will keep them anesthetized. Once the flies are in the correct orientation for your experiment, proceed with the injections. In the example protocol, we will inject adult flies with fluorescein-labeled particles for in vivo imaging.

- After pulling thin-walled glass capillaries with a needle puller, use a micrometer to hold the needle under a microscope and use number five fine-point stainless steel tweezers to break