Drosophila Egg Collection and Dechorionation: een methode om de buitenste eilaag te verwijderen

- Allow Drosophila to lay eggs overnight onto a dish containing agar supplemented with grape juice to visualize eggs and coated with yeast to encourage flies to mate and lay eggs. To remove the eggs, add distilled water to the dish and gently wipe the surface with a paintbrush. Then pour the mixture through a nylon mesh placed in a plastic bushing to collect the eggs. Soak the eggs in two rounds of 7% bleach to remove the chorion, or protective outer layer, from the embryo. This process is called dechorionation.

The eggs are now stuck to the side of the bushing. Wash them three times with water to remove the bleach. Finally, use a paintbrush to transfer the eggs from the bushing to vials to continue development or for immediate use in an experiment. In the example protocol, we will collect and dechorionate Drosophila eggs under sterile conditions to rear flies with defined microbiota.

- Place nylon mesh into a plastic bushing to prepare the sieve for egg collection. If the same flies will be used the next day, immediately transfer them to a new cage containing freshly yeasted grape juice agar plates. The grape juice plate in the previous cage contains the fruit fly eggs. Remove dea