Micro-injection van Levende Embryo's Drosophila: Vroege Levering van Reagentia aan het Ontwikkelende Embryo

- Collect live Drosophila embryos and remove their non-transparent chorions. Then, onto a glass coverslip, apply a line of heptane glue, which is tape adhesive dissolved in liquid heptane. To be able to handle the now dechorionated embryos, carefully align the specimens in a row onto the adhesive.

Transfer the coverslip into a dehydration chamber to partially desiccate the embryos. This will prevent cytoplasmic leakage during or after injections. Next, remove the coverslip and cover the embryos with halocarbon oil to prevent further dehydration while still allowing oxygen to diffuse into the embryos.

To perform the microinjection, use a microinjection system on a compatible microscope. By looking through the eyes pieces, identify both the embryos and the needle preloaded with injection solution. Break open the needle and ensure that it produces consistent and appropriately-sized liquid drops. Then, bring the needle inside the embryo and inject the appropriate volume. Bring out the needle, and proceed to the next one, repeating the injection for the remaining embryos.

In the example protocol, we will see a demonstration of Drosophila embryo microinjection for live imaging studies of