Hele genoom Sequencing van Candida glabrata voor detectie van Markers van antischimmel drugweerstand

12.1K weergaven

Geciteerd door 22

08:45 min.

28 december 2017

10.3791/56714-v

28 december 2017

12.1K weergaven

Chayanika Biswas^*¹ , Sharon C-A. Chen^*¹^,²^,³ , Catriona Halliday¹^,² , Elena Martinez¹ , Rebecca J. Rockett¹ , Qinning Wang¹ , Verlaine J. Timms¹ , Rajat Dhakal¹ , Rosemarie Sadsad¹ , Karina J. Kennedy⁴ , Geoffrey Playford⁴^,⁵ , Deborah J. Marriott⁶ , Monica A. Slavin⁷ , Tania C. Sorrell¹^,³ , Vitali Sintchenko¹^,²^,³

¹Centre for Infectious Diseases and Microbiology-Public Health, Westmead Hospital, ²Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, ³Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, ⁴Department of Microbiology and Infectious Diseases, Canberra Hospital and Health Services, Australian National University Medical School, ⁵Infection Management Services, Australian National University Medical School, ⁶Department of Microbiology and Infectious Diseases, St. Vincent's Hospital, ⁷Department of Infectious Diseases, Peter MacCallum Cancer Centre

^* These authors contributed equally

Deze studie uitgevoerd hele genoom sequencing p.a. van mutaties in genen antischimmel medicamenteuse resistentie bij Candida glabratatoekennen. C. glabrata isolaten resistent tegen echinocandins, azoles en 5-flucytosine, werden sequenced ter illustratie van de methodologie. De profielen van de gevoeligheid van de isolaten gecorreleerd met aanwezigheid of afwezigheid van bepaalde mutatie patronen in de genen.