Name: Onderzoek naar alternatieve perfusieoplossingen met behulp van gepolymeriseerde zuurstofdragers op basis van hemoglobine van de volgende generatie in een model van ex vivo longperfusie bij ratten (Video) | JoVE Journal
Uploaded: 2024-06-14T14:10:00.000+00:00
Duration: 9 min 47 s
Description: Ontdek de toepassing van geïndustrialiseerde hemoglobine als zuurstofdrager in een rattenmodel voor ex vivo longperfusie. Deze methode verbetert de evaluatie van donorlongen en kan de beschikbaarheid van donororganen vergroten.

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<meta charset="utf8"></head><body>Longtransplantatie bevorderen met op polyhHb gebaseerde perfusie in EVLP-modellen voor ratten

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<li>Gouchoe, D. A., Whitson, B. A., Zhu, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+next+frontier+in+lung+transplantation%3A+Protecting+the+endothelium+and+repairing+organs+for+transplant+utilizing+MG53%5BTitle%5D)+AND+%22Clin+Transl+Dis%22%5BJournal%5D)">The next frontier in lung transplantation: Protecting the endothelium and repairing organs for transplant utilizing MG53.</a> Clin Transl Dis. 3 (6), e255(2023).</li>
<li>Gouchoe, D. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((MG53+mitigates+warm+ischemic+lung+injury+in+a+murine+model+of+transplantation%5BTitle%5D)+AND+%22J+Thorac+Cardiovasc+Surg%22%5BJournal%5D)">MG53 mitigates warm ischemic lung injury in a murine model of transplantation.</a> J Thorac Cardiovasc Surg. , 10.1016/j.jtcvs.2023.10.056 (2023).</li>
</ol>

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1879</td><td></td></tr><tr><td>Expired human leukoreduced, packed RBC units</td><td>Wexner Medical Center Canadian Blood Services Zen-Bio Inc</td><td></td><td></td></tr><tr><td>Fiberoxygenator D150</td><td>Hugo Sachs Elektronik</td><td>PY2 73-3762</td><td></td></tr><tr><td>Forceps</td><td>Fine Science tools</td><td>11027-12</td><td></td></tr><tr><td>Glutaraldehyde (C5H8O2 70 wt%)</td><td>Sigma Aldrich</td><td>111-30-8 (G7776)</td><td></td></tr><tr><td>Halsted-Mosquito Hemostat</td><td>Roboz Surgical</td><td>RS-7112</td><td></td></tr><tr><td>Heparin 30,000 units per 30 ml</td><td>APP Pharmaceuticals</td><td></td><td></td></tr><tr><td>Human Serum Albumin (HSA)</td><td>OctaPharma Plasma</td><td></td><td>Perfusate toevoeging</td></tr><tr><td>IL2 Tube set for perfusate</td><td>Harvard Apparatus</td><td>733842</td><td></td></tr><tr><td>IPL-2 Basic Lung Perfusion System</td><td>Harvard Apparatus</td><td></td><td></td></tr><tr><td>Ketamine 500 mg per 5 ml</td><td>JHP Pharmaceuticals</td><td></td><td></td></tr><tr><td>Left Atrium cannula</td><td>Harvard Apparatus</td><td>730712</td><td></td></tr><tr><td>Liqui-Cel EXF Series G420 Membrane Contactor</td><td>3M</td><td>G420</td><td>gas contactor</td></tr><tr><td>low potassium dextran glucose solution (perfadex)</td><td>XVIVO</td><td></td><td>oplossing voor het spoelen van de long</td></tr><tr><td>Masterflex Platinum Coated Tubing(Size: 73,17,16,24)</td><td>Cole-Palmer</td><td></td><td></td></tr><tr><td>N-Acetyl-L-cysteine (NALC, C5H9NO3S)</td><td>Sigma Aldrich</td><td>616-91-1 (A7250)</td><td>Voor gemodificeerd Ringer's lactate</td></tr><tr><td>Nalgene Vessels (10L, 20L)</td><td>Nalgene</td><td></td><td>Filtratie vaten</td></tr><tr><td>Peristaltic Pump</td><td>&nbsp;Ismatec</td><td>&nbsp;ISM 827B</td><td></td></tr><tr><td>PES, 0.65 &micro;m TFF module</td><td>Repligen</td><td>N02-E65U-07-N</td><td></td></tr><tr><td>PhysioSuite</td><td>Kent Scientific Corporation</td><td>PS-MSTAT-RT</td><td></td></tr><tr><td>polyethersulfone (PES), 0.2 &micro;m TFF module</td><td>Repligen</td><td>N02-S20U-05-N</td><td></td></tr><tr><td>Polysulfone (PS), 500 kDa TFF module</td><td>Repligen</td><td>N02-P500-05-N</td><td></td></tr><tr><td>Potassium Chloride (KCl)</td><td>Fisher Scientific</td><td>7447-40-7</td><td>Voor PBS</td></tr><tr><td>PowerLab 8/35</td><td>&nbsp;ADInstruments</td><td>730045</td><td></td></tr><tr><td>Pulmonary Artery cannula</td><td>Harvard Apparatus</td><td>730710</td><td></td></tr><tr><td>Pump Head tubing (Size: 73,17,16,24)</td><td>PharMed BPT</td><td></td><td></td></tr><tr><td>Puralube&nbsp;Ophthalmic Ointment</td><td>Dechra</td><td>NA</td><td></td></tr><tr><td>Scissors</td><td>Fine Science tools</td><td>14090-11</td><td></td></tr><tr><td>SCP Servo controller for perfusion type 704</td><td>Harvard Apparatus</td><td>732806</td><td></td></tr><tr><td>Small Animal Ventilator model 683</td><td>Harvard Apparatus</td><td>55-000</td><td></td></tr><tr><td>Sodium Chloride (NaCl)</td><td>Fisher Scientific</td><td>7647-14-5 (S271-10)</td><td>Voor PBS en zoutoplossing</td></tr><tr><td>Sodium cyanoborohydride (NaCNBH3)</td><td>Sigma Aldrich</td><td>25895-60-7</td><td></td></tr><tr><td>Sodium Dithionite (Na2S2O4)</td><td>Sigma Aldrich</td><td>7775-14-6</td><td></td></tr><tr><td>Sodium Hydroxide (NaOH)</td><td>Fisher Scientific</td><td>1310-73-2</td><td>Voor gemodificeerd Ringer's lactate</td></tr><tr><td>Sodium Lactate (NaC3H5O3)</td><td>Sigma Aldrich</td><td>867-56-1</td><td>Voor gemodificeerd Ringer's lactate</td></tr><tr><td>Sodium phosphate dibasic (Na2HPO4)</td><td>Fisher Scientific</td><td>7558-79-4</td><td>Voor PBS</td></tr><tr><td>Sodium phosphate monobasic (NaH2PO4)</td><td>Fisher Scientific</td><td>7558-80-7</td><td>Voor PBS</td></tr><tr><td>SomnoSuite Small Animal Anesthesia System</td><td>Kent Scientific Corporation</td><td>SS-MVG-Module</td><td></td></tr><tr><td>Sprague-Dawley rats</td><td>Envigo</td><td></td><td></td></tr><tr><td>TAM-A transducer amplifier module type 705/1</td><td>Harvard Apparatus</td><td>73-0065</td><td></td></tr><tr><td>TAM-D transducer amplifier type 705/2</td><td>Harvard Apparatus</td><td>&nbsp;73-1793</td><td></td></tr><tr><td>TCM time control module type 686</td><td>Harvard Apparatus</td><td>731750</td><td></td></tr><tr><td>Tracheal cannula</td><td>Harvard Apparatus</td><td>733557</td><td></td></tr><tr><td>Tube set for moist chamber</td><td>Harvard Apparatus</td><td>&nbsp;73V83157</td><td></td></tr><tr><td>Tubing Cassette</td><td>Cole-Parmer</td><td>IS 0649</td><td></td></tr><tr><td>Tweezer #5 Dumostar</td><td>Kent Scientific Corporation</td><td>&nbsp;INS500085-A</td><td></td></tr><tr><td>Tweezer #5 stainless steel, curved</td><td>Kent Scientific Corporation</td><td>IND500232</td><td></td></tr><tr><td>Tweezer #7 Titanium</td><td>Kent Scientific Corporation</td><td>&nbsp;INS600187</td><td></td></tr><tr><td>Tygon E-3603 Tubing 2.4 mm ID</td><td>Harvard Apparatus</td><td>721017</td><td>perfusate lijn die de long binnenkomt</td>

onderzoek naar alternatieve perfusieoplossingen met behulp van gepolymeriseerde zuurstofdragers op basis van hemoglobine van de volgende generatie in een model van ex vivo longperfusie bij ratten

Exploring Alternative Perfusion Solutions Using Next-Generation Polymerized Hemoglobin-Based Oxygen Carriers in a Model of Rat Ex Vivo Lung Perfusion

Onderzoek naar alternatieve perfusieoplossingen met behulp van gepolymeriseerde zuurstofdragers op basis van hemoglobine van de volgende generatie in een model van ex vivo longperfusie bij ratten

Lung transplantation is hampered by the lack of suitable donors. Previously, donors that were thought to be marginal or inadequate were discarded. ...

exploring-alternative-perfusion-solutions-using-next-generation

Research

JoVE Journal

Medicine

Geneeskunde

Ex-Vivo longperfusie (EVLP) heeft toegestaan ​​longtransplantatie bij de mens om meer direct beschikbaar doordat de mogelijkheid om organen te beoordelen en uit te breiden het donorpool geworden. We beschrijven hier de ontwikkeling van een rat EVLP programma en verfijningen die zorgen voor een reproduceerbaar model voor toekomstige uitbreiding.

WERKWIJZE Geïsoleerde<em&gt; Ex Vivo</em&gt; Longperfusie in een Rat Model: Lessen uit het ontwikkelen van een Rat EVLP Program

Dit document wordt beschreven hoe u cardiopulmonale bypass bij muizen. Dit nieuwe model zal het vergemakkelijken van het onderzoek in de moleculaire mechanismen die betrokken zijn bij orgel schade.

Cardiopulmonale Bypass in een muismodel: een nieuwe benadering

Er is een belangrijke donor van lever tekort, en criteria voor lever donoren hebben uitgebreid. Normothermic ex vivo lever perfusie (NEVLP) is ontwikkeld om te evalueren en wijzigen van orgaanfunctie. Deze studie toont een model van de rat van NEVLP en test het vermogen van gepegyleerde-katalase, te verzachten lever behoud letsel.

Een kleine diermodel van Ex Vivo Normothermic lever perfusie

Het geïsoleerde konijnenlongpreparaat is een gouden standaardinstrument in longonderzoek. Deze publicatie heeft tot doel de techniek te beschrijven zoals ontwikkeld voor de studie van fysiologische en pathologische mechanismen die betrokken zijn bij luchtwegreactiviteit, longbehoud en preklinisch onderzoek bij longtransplantatie en longoedeem.

Geïsoleerd longperfusiesysteem in het konijnenmodel

Ex vivo longen zijn nuttig voor een verscheidenheid aan experimenten om fysiologische gegevens te verzamelen, terwijl de verstorende variabelen van in vivo experimenten worden uitgesloten. Commerciële opstellingen zijn vaak duur en beperkt in de soorten gegevens die ze kunnen verzamelen. We beschrijven een methode voor het bouwen van een volledig modulaire opstelling, aanpasbaar voor verschillende onderzoeksontwerpen.

Ontwerp en implementatie van een ex vivo longperfusiemodel voor ratten

Hier presenteren we een beoordelingsprotocol van een heterotopisch geïmplanteerd hart na normotherm ex situ behoud in het rattenmodel.

Rattenmodel van Normothermische ex-situ perfuseerde heterotope harttransplantatie

Een normotherm ex vivo leverperfusie (NEVLP) systeem werd gecreëerd voor muizenlevers. Dit systeem vereist ervaring in microchirurgie, maar zorgt voor reproduceerbare perfusieresultaten. Het vermogen om muizenlevers te gebruiken vergemakkelijkt het onderzoek van moleculaire routes om nieuwe perfusaatadditieven te identificeren en maakt de uitvoering van experimenten gericht op orgaanherstel mogelijk.

Normothermic Ex Vivo Lever Machine Perfusie in Muis

Hier beschrijven we de noodzakelijke stappen voor het opstellen van een EVLP-model bij ratten en tonen we het ontstekingsprofiel dat verband houdt met de geperfuseerde longen. Het doel is om kennis en ervaringen over het EVLP-model van ratten te verspreiden, waardoor een integraal begrip mogelijk wordt van de biologische reacties die met die revolutionaire techniek gepaard gaan.

Opstelling van een ex vivo longperfusiemodel bij ratten voor translationele inzichten in longtransplantatie

Het doel is om een nieuw ex vivo longperfusie (EVLP) model bij ratten te ontwikkelen dat 4 uur lang een consistente longfunctie kan handhaven gedurende EVLP en de industrie te voorzien van implementatiedetails die een stevige methode bieden voor het toepassen van EVLP op knaagdiermodellen.

Oprichting van een nieuw Ex Vivo longperfusiesysteem voor rattenlongen na circulatoire dood

Marginale enten, zoals vette levers, enten van oudere donoren, of levers teruggehaald na hartdood (DCD) tolereren conventionele, koude statische opslag alleen slecht. We ontwikkelden een nieuw model van subnormothermic ex vivo lever perfusie voor het behoud, de beoordeling, en de reparatie van marginale lever transplantaties voorafgaand aan de transplantatie.

Techniek van Subnormothermic<em&gt; Ex Vivo</em&gt; Leverperfusie voor de opslag, Assessment, en reparatie van Marginal Lever Enten

Ontdek de toepassing van geïndustrialiseerde hemoglobine als zuurstofdrager in een rattenmodel voor ex vivo longperfusie. Deze methode verbetert de evaluatie van donorlongen en kan de beschikbaarheid van donororganen vergroten.

Onderzoek naar alternatieve perfusieoplossingen met behulp van gepolymeriseerde zuurstofdragers op basis van hemoglobine van de volgende generatie in een model van ex vivo longperfusie bij ratten (Video) | JoVE Journal

Onderzoek naar alternatieve perfusieoplossingen met behulp van gepolymeriseerde zuurstofdragers op basis van hemoglobine van de volgende generatie in een model van ex vivo longperfusie bij ratten

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