Name: Opstellen van in-vitromodellen van dorsale wortelgangliacultuur: complementaire benaderingen voor het onderzoeken van overspraak tussen kanker en zenuw (Video) | JoVE Journal
Uploaded: 2025-07-11T16:10:00.000+00:00
Duration: 11 min 26 s
Description: Leer hoe je 3D- en 2D-dorsale wortelgangliacultures opzet uit één muis om tumor-zenuwinteracties te bestuderen. Deze methode optimaliseert neurietgroei en kwantificeert neuro-geleide kankermigratie.

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<ol><li>Thiel, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+single+neurons+reprogrammed+by+pancreatic+cancer%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Characterization of single neurons reprogrammed by pancreatic cancer.</a> Nature. 640 (8060), 1042-1051 (2025).</li>
<li>Zhu, M., Luo, F., Xu, B., Xu, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Research+progress+of+neural+invasion+in+pancreatic+cancer%5BTitle%5D)+AND+%22Curr+Cancer+Drug+Targets%22%5BJournal%5D)">Research progress of neural invasion in pancreatic cancer.</a> Curr Cancer Drug Targets. 24 (4), 397-410 (2024).</li>
<li>Zareba, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perineural+invasion+and+risk+of+lethal+prostate+cancer%5BTitle%5D)+AND+%22Cancer+Epidemiol+Biomarkers+Prev%22%5BJournal%5D)">Perineural invasion and risk of lethal prostate cancer.</a> Cancer Epidemiol Biomarkers Prev. 26 (5), 719-726 (2017).</li>
<li>Fu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Worst+pattern+of+perineural+invasion+redefines+the+spatial+localization+of+nerves+in+oral+squamous+cell+carcinoma%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Worst pattern of perineural invasion redefines the spatial localization of nerves in oral squamous cell carcinoma.</a> Front Oncol. 11 (766902), (2021).</li>
<li>Hu, J., Chen, W., Shen, L., Chen, Z., Huang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Crosstalk+between+the+peripheral+nervous+system+and+breast+cancer+influences+tumor+progression%5BTitle%5D)+AND+%22Biochim+Biophys+Acta+Rev+Cancer%22%5BJournal%5D)">Crosstalk between the peripheral nervous system and breast cancer influences tumor progression.</a> Biochim Biophys Acta Rev Cancer. 1877 (6), 188828(2022).</li>
<li>Li, J., Mei, S., Zhou, S., Zhao, F., Liu, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perineural+invasion+is+a+prognostic+factor+in+stage+ii+colorectal+cancer+but+not+a+treatment+indicator+for+traditional+chemotherapy%3A+A+retrospective+cohort+study%5BTitle%5D)+AND+%22J+Gastrointest+Oncol%22%5BJournal%5D)">Perineural invasion is a prognostic factor in stage ii colorectal cancer but not a treatment indicator for traditional chemotherapy: A retrospective cohort study.</a> J Gastrointest Oncol. 13 (2), 710-721 (2022).</li>
<li>Schmitd, L. B., Perez-Pacheco, C., D'silva, N. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nerve+density+in+cancer%3A+Less+is+better%5BTitle%5D)+AND+%22FASEB+Bioadv%22%5BJournal%5D)">Nerve density in cancer: Less is better.</a> FASEB Bioadv. 3 (10), 773-786 (2021).</li>
<li>Zahalka, A. H., Frenette, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nerves+in+cancer%5BTitle%5D)+AND+%22Nat+Rev+Cancer%22%5BJournal%5D)">Nerves in cancer.</a> Nat Rev Cancer. 20 (3), 143-157 (2020).</li>
<li>Winkler, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neuroscience+and+oncology%3A+State-of-the-art+and+new+perspectives%5BTitle%5D)+AND+%22Curr+Opin+Neurol%22%5BJournal%5D)">Neuroscience and oncology: State-of-the-art and new perspectives.</a> Curr Opin Neurol. 36 (6), 544-548 (2023).</li>
<li>Lanigan, L. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparative+pathology+of+the+peripheral+nervous+system%5BTitle%5D)+AND+%22Vet+Pathol%22%5BJournal%5D)">Comparative pathology of the peripheral nervous system.</a> Vet Pathol. 58 (1), 10-33 (2021).</li>
<li>Jiang, S. -H., Zhang, S., Wang, H., Xue, J. -L., Zhang, Z. -G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Emerging+experimental+models+for+assessing+perineural+invasion+in+human+cancers%5BTitle%5D)+AND+%22Cancer+Lett%22%5BJournal%5D)">Emerging experimental models for assessing perineural invasion in human cancers.</a> Cancer Lett. 535 (215610), (2022).</li>
<li>Moysidou, C. -M., Barberio, C., Owens, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advances+in+engineering+human+tissue+models%5BTitle%5D)+AND+%22Front.+Bioeng.+Biotechnol%22%5BJournal%5D)">Advances in engineering human tissue models.</a> Front. Bioeng. Biotechnol. 8 (620962), (2021).</li>
<li>Bayat, F. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adult+mouse+dorsal+root+ganglia+neurons+form+aberrant+glutamatergic+connections+in+dissociated+cultures%5BTitle%5D)+AND+%22PloS+ONE%22%5BJournal%5D)">Adult mouse dorsal root ganglia neurons form aberrant glutamatergic connections in dissociated cultures.</a> PloS ONE. 16 (3), e0246924(2021).</li>
<li>Lin, Y. -T., Chen, J. -C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dorsal+root+ganglia+isolation+and+primary+culture+to+study+neurotransmitter+release%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Dorsal root ganglia isolation and primary culture to study neurotransmitter release.</a> J Vis Exp. (140), e57569(2018).</li>
<li>Schmidt, H., Rathjen, F. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dii-labeling+of+drg+neurons+to+study+axonal+branching+in+a+whole+mount+preparation+of+mouse+embryonic+spinal+cord%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Dii-labeling of drg neurons to study axonal branching in a whole mount preparation of mouse embryonic spinal cord.</a> J Vis Exp. (58), e3667(2011).</li>
<li>Klimovich, P., Rubina, K., Sysoeva, V., Semina, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+model+of+dorsal+root+ganglion+explant+as+a+method+of+studying+neurotrophic+factors+in+regenerative+medicine%5BTitle%5D)+AND+%22Biomedicines%22%5BJournal%5D)">Three-dimensional model of dorsal root ganglion explant as a method of studying neurotrophic factors in regenerative medicine.</a> Biomedicines. 8 (3), 49(2020).</li>
<li>Neto, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Axonal+outgrowth%2C+neuropeptides+expression+and+receptors+tyrosine+kinase+phosphorylation+in+3d+organotypic+cultures+of+adult+dorsal+root+ganglia%5BTitle%5D)+AND+%22PLoS+ONE%22%5BJournal%5D)">Axonal outgrowth, neuropeptides expression and receptors tyrosine kinase phosphorylation in 3d organotypic cultures of adult dorsal root ganglia.</a> PLoS ONE. 12 (7), e0181612(2017).</li>
<li>Melli, G., Höke, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dorsal+root+ganglia+sensory+neuronal+cultures%3A+A+tool+for+drug+discovery+for+peripheral+neuropathies%5BTitle%5D)+AND+%22Expert+Opin+Drug+Discov%22%5BJournal%5D)">Dorsal root ganglia sensory neuronal cultures: A tool for drug discovery for peripheral neuropathies.</a> Expert Opin Drug Discov. 4 (10), 1035-1045 (2009).</li>
<li>Du, J., Sudlow, L. C., Luzhansky, I. D., Berezin, M. Y. Drg explant model: Elucidating mechanisms of oxaliplatin-induced peripheral neuropathy and identifying potential therapeutic targets. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aDu%2C+J+%22bioRxiv%22">bioRxiv</a>. , (2023).</li>
<li>Mo, Y. J., Kim, Y. -S., Kim, M. S., Lee, Y. -I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advantages+of+adult+mouse+dorsal+root+ganglia+explant+culture+in+investigating+myelination+in+an+inherited+neuropathic+mice+model%5BTitle%5D)+AND+%22Methods+Protoc%22%5BJournal%5D)">Advantages of adult mouse dorsal root ganglia explant culture in investigating myelination in an inherited neuropathic mice model.</a> Methods Protoc. 5 (4), 66(2022).</li>
<li>Nguyen, U. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Type+i+collagen+concentration+affects+neurite+outgrowth+of+adult+rat+drg+explants+by+altering+mechanical+properties+of+hydrogels%5BTitle%5D)+AND+%22J+Biomater+Sci+Polym+Ed%22%5BJournal%5D)">Type i collagen concentration affects neurite outgrowth of adult rat drg explants by altering mechanical properties of hydrogels.</a> J Biomater Sci Polym Ed. 35 (2), 164-189 (2024).</li>
<li>Ito, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Histological+effects+of+combined+therapy+involving+scar+resection%2C+decellularized+scaffolds%2C+and+human+ipsc-ns%2Fpcs+transplantation+in+chronic+complete+spinal+cord+injury%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Histological effects of combined therapy involving scar resection, decellularized scaffolds, and human ipsc-ns/pcs transplantation in chronic complete spinal cord injury.</a> Sci Rep. 14 (1), (2024).</li>
<li>Aisenbrey, E. A., Murphy, W. L., Aisenbrey, E. A., Murphy, W. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Synthetic+alternatives+to+matrigel%5BTitle%5D)+AND+%22Nat+Rev+Mater%22%5BJournal%5D)">Synthetic alternatives to matrigel.</a> Nat Rev Mater. 5 (7), 539-551 (2020).</li>
<li>Berkovitch, Y., Seliktar, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Semi-synthetic+hydrogel+composition+and+stiffness+regulate+neuronal+morphogenesis+-+pubmed%5BTitle%5D)+AND+%22Int+J+Pharm%22%5BJournal%5D)">Semi-synthetic hydrogel composition and stiffness regulate neuronal morphogenesis - pubmed.</a> Int J Pharm. 523 (2), 545-555 (2017).</li>
<li>Carozzi, V. A., Salio, C., Rodriguez Menendez, V., Ciglieri, E., Ferrini, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((2d+vs+3d+morphological+analysis+of+dorsal+root+ganglia+in+health+and+painful+neuropathy%5BTitle%5D)+AND+%22Eur+J+Histochem%22%5BJournal%5D)">2d vs 3d morphological analysis of dorsal root ganglia in health and painful neuropathy.</a> Eur J Histochem. 65 (Suppl 1), 3276(2021).</li>
<li>Smith, P. R., Meyer, A., Loerch, S., Campbell, Z. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protocol+for+the+isolation+and+culture+of+mouse+dorsal+root+ganglion+neurons+for+imaging+applications%5BTitle%5D)+AND+%22STAR+Protocols%22%5BJournal%5D)">Protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications.</a> STAR Protocols. 4 (4), (2023).</li>
<li>Bunge, R. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Expanding+roles+for+the+schwann+cell%3A+Ensheathment%2C+myelination%2C+trophism+and+regeneration+-+pubmed%5BTitle%5D)+AND+%22Curr+Opin+Neurobiol%22%5BJournal%5D)">Expanding roles for the schwann cell: Ensheathment, myelination, trophism and regeneration - pubmed.</a> Curr Opin Neurobiol. 3 (5), 805-809 (1993).</li>
<li>Chen, Z., Fang, Y., Jiang, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Important+cells+and+factors+from+tumor+microenvironment+participated+in+perineural+invasion%5BTitle%5D)+AND+%22Cancers%22%5BJournal%5D)">Important cells and factors from tumor microenvironment participated in perineural invasion.</a> Cancers. 15 (5), 1360(2023).</li>
<li>Jang, K., Garraway, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+review+of+dorsal+root+ganglia+and+primary+sensory+neuron+plasticity+mediating+inflammatory+and+chronic+neuropathic+pain%5BTitle%5D)+AND+%22Neurobiol+Pain%22%5BJournal%5D)">A review of dorsal root ganglia and primary sensory neuron plasticity mediating inflammatory and chronic neuropathic pain.</a> Neurobiol Pain. 15 (100151), (2024).</li>
<li>Hanani, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-related+changes+in+neurons+and+satellite+glial+cells+in+mouse+dorsal+root+ganglia%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Age-related changes in neurons and satellite glial cells in mouse dorsal root ganglia.</a> Int J Mol Sci. 24 (3), 2677(2023).</li>
<li>Martinelli, C., Sartori, P., Ledda, M., Pannese, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-related+quantitative+changes+in+mitochondria+of+satellite+cell+sheaths+enveloping+spinal+ganglion+neurons+in+the+rabbit%5BTitle%5D)+AND+%22Brain+Res+Bull%22%5BJournal%5D)">Age-related quantitative changes in mitochondria of satellite cell sheaths enveloping spinal ganglion neurons in the rabbit.</a> Brain Res Bull. 61 (2), 147-151 (2003).</li>
<li>Niwa, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+age-dependent+trophic+responses+of+nodose%2C+sensory%2C+and+sympathetic+neurons+to+neurotrophins+and+gdnf%3A+Potencies+for+neurite+extension+in+explant+culture%5BTitle%5D)+AND+%22Neurochem+Res%22%5BJournal%5D)">Differential age-dependent trophic responses of nodose, sensory, and sympathetic neurons to neurotrophins and gdnf: Potencies for neurite extension in explant culture.</a> Neurochem Res. 27 (6), 485-496 (2002).</li>
<li>Adler, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-dependent+differential+regulation+of+sensory+neuropeptides+by+glial+cell+line-derived+neurotrophic+factor%5BTitle%5D)+AND+%22J+Neurochem%22%5BJournal%5D)">Age-dependent differential regulation of sensory neuropeptides by glial cell line-derived neurotrophic factor.</a> J Neurochem. 71 (1), 170-177 (1998).</li>
<li>Podratz, J. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neurotoxicity+to+drg+neurons+varies+between+rodent+strains+treated+with+cisplatin+and+bortezomib%5BTitle%5D)+AND+%22J+Neurol+Sci%22%5BJournal%5D)">Neurotoxicity to drg neurons varies between rodent strains treated with cisplatin and bortezomib.</a> J Neurol Sci. 362, 131-135 (2015).</li>
<li>Lawson, S. N., Biscoe, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+mouse+dorsal+root+ganglia%3A+An+autoradiographic+and+quantitative+study%5BTitle%5D)+AND+%22J+Neurocytol%22%5BJournal%5D)">Development of mouse dorsal root ganglia: An autoradiographic and quantitative study.</a> J Neurocytol. 8 (3), 265-274 (1979).</li>
<li>Monje, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Roadmap+for+the+emerging+field+of+cancer+neuroscience%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Roadmap for the emerging field of cancer neuroscience.</a> Cell. 181 (2), 219-222 (2020).</li>
<li>Silverman, D. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cancer-associated+neurogenesis+and+nerve-cancer+cross-talk%5BTitle%5D)+AND+%22Cancer+Res%22%5BJournal%5D)">Cancer-associated neurogenesis and nerve-cancer cross-talk.</a> Cancer Res. 81 (6), 1431-1440 (2021).</li>
<li>Vermeer, P. D., Restaino, A. C., Barr, J. L., Yaniv, D., Amit, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nerves+at+play%3A+The+peripheral+nervous+system+in+extracranial+malignancies%5BTitle%5D)+AND+%22Cancer+Discov%22%5BJournal%5D)">Nerves at play: The peripheral nervous system in extracranial malignancies.</a> Cancer Discov. 15 (1), 52-68 (2025).</li>
</ol>

<table><tbody><tr><td>Hulpmiddelen</td><td></td><td></td><td></td></tr><tr><td>70 &micro;m Cell Strainer</td><td>Progene</td><td>71-229483-ULT</td><td>Gebalikt voor het filteren van celsuspensie</td></tr><tr><td>8-well glazen bodem dia's</td><td>IBidi, München, Duitsland</td><td>80807</td><td></td></tr><tr><td>Dumont #5 - Fijne pincet</td><td>Fine Science Tools, Foster City, USA</td><td>11254-20</td><td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td>Dumont #5 pincet</td><td>Fine Science Tools, Foster City, USA</td><td>11252-20</td><td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td>Fijne schaar</td><td>Fine Science Tools, Foster City, USA</td><td>91460-11</td><td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td>Patronen pincet</td><td>Fine Science Tools, Foster City, USA</td><td>91121-12</td><td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td>Veder schaar</td><td>Fine Science Tools, Foster City, USA</td><td>91500-09</td><td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td>Reagentia</td><td></td><td></td><td></td></tr><tr><td>1x Fosfaat gebufferde zoutoplossing</td><td>Sigma, St. Louis, USA</td><td>D8662-500ML</td><td>Gebalikt voor het oplossen van enzymen</td></tr><tr><td>B-27 Supplement (50x)</td><td>Thermo Fisher Scientific, Massachusetts, USA</td><td>17504044</td><td>Gebalikt bij 2% concentratie in F12 media</td></tr><tr><td>Bovine Type I Collageen</td><td>Corning Incorporated, New York, USA</td><td>354231</td><td>Gebalikt voor het coaten van platen, werkoplossing 50 &micro;g/mL in 0,01 M HCl</td></tr><tr><td>Collageenase Type IV</td><td>Sigma-Aldrich, St. Louis, USA</td><td>C4-BIOC</td><td>Oplossen in PBS bij 1,25 mg/mL, verdelen in aliquoten, bewaar bij -20 &deg;C</td></tr><tr><td>Dispase II</td><td>Roche, Bazel, Zwitserland</td><td>4942078001</td><td>Oplossen in PBS bij 2,5 mg/mL, verdelen in aliquoten, bewaar bij -20 &deg;C</td></tr><tr><td>DNase I</td><td>Roche, Basel, Zwitserland.</td><td>11284932001</td><td>Toegevoegd bij een uiteindelijke concentratie van&nbsp; 0,2 mg/mL tijdens dissociatie</td></tr><tr><td>Fetal Bovine Serum (FBS)</td><td>Sigma-Aldrich, St. Louis, USA</td><td>F1051</td><td>Gebalikt bij 10% concentratie in F12 media</td></tr><tr><td>HBSS (Hank's Balanced Salt Solution)</td><td>Sigma, St. Louis, USA</td><td>H6648-500ML</td><td>Bereid met 1% Penicilline-Streptomycine</td></tr><tr><td>Matrigel Growth Factor Reduced</td><td>Corning Incorporated, New York, USA</td><td>356231</td><td>Ontdooi bij 4 &deg;C overnacht, houd op ijs</td></tr><tr><td>Nutrient Mixture F-12 Ham</td><td>Sigma-Aldrich, St. Louis, USA</td><td>N6658-500ML</td><td>Basis medium voor bereiding van aangevuld kweekmedium</td></tr><tr><td>Papaïne</td><td>Roche, Bazel, Zwitserland.</td><td>10108014001</td><td>Oplossen in PBS bij 30 U/mL, verdelen in aliquoten, bewaar bij -20 &deg;C</td></tr><tr><td>Penicilline-Streptomycine (100x)</td><td>Sigma-Aldrich, St. Louis, USA</td><td>P4333-100ML</td><td>Gebalikt bij 1% concentratie in media</td></tr><tr><td>Trypan Blauw Oplossing</td><td>Sigma-Aldrich, St. Louis, USA</td><td>T8154-100ML</td><td>Om celviabiliteit te beoordelen</td></tr><tr><td>TrypLE Express</td><td>Thermo Fisher Scientific, Massachusetts, USA</td><td>12605-028</td><td>Voor het oogsten van kankercellen</td></tr><tr><td>Antilichamen</td><td></td><td></td><td></td></tr><tr><td>&beta;-Tubuline III antilichaam</td><td>Abcam (Cambridge, UK)</td><td>AB15568</td><td>Verdunning 1:200</td></tr><tr><td>Alexa Fluor 488</td><td>Thermo Fisher Scientific, Massachusetts, USA</td><td>A-11008</td><td>Verdunning 1:2000</td></tr><tr><td>Alexa Fluor 594</td><td>Thermo Fisher Scientific, Massachusetts, USA</td><td>A-21442</td><td>Verdunning 1:2000</td></tr><tr><td>Alexa Fluor 647</td><td>Thermo Fisher Scientific, Massachusetts, USA</td><td>A-21245</td><td>Verdunning 1:2000</td></tr><tr><td>NeuN antilichaam</td><td>Sigma-Aldrich, St. Louis, USA</td><td>MAB377</td><td>Verdunning 1:100</td></tr><tr><td>Fluorescente kleurstoffen &amp; sondes</td><td></td><td></td><td></td></tr><tr><td>ActinGreen 488 ReadyProbes Reagens (Faloïdin)</td><td>Thermo Fisher Scientific, Massachusetts, USA</td><td>R37110</td><td>Verdunning 1:40</td></tr><tr><td>CellTrace Calcein Red-Orange</td><td>Thermo Fisher Scientific, Massachusetts, USA</td><td>C34851</td><td>Celtracker gebruikt om neuronen onder fluorescentiemicroscoop te visualiseren</td></tr><tr><td>Hoechst 33342</td><td>Thermo Fisher Scientific, Massachusetts, USA</td><td>H3570</td><td>Verdunning 1:5000</td></tr></tbody></table>

opstellen van in-vitromodellen van dorsale wortelgangliacultuur: complementaire benaderingen voor het onderzoeken van overspraak tussen kanker en zenuw

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Modeling Cancerous Neural Invasion In Vitro: A Method to Co-culture Dorsal Root Ganglia and Cancer Cells

Fenotypisch wild-type astrocyten en neurale stamcellen die zijn geoogst uit muizen die zijn gemodificeerd met gefloxte, conditionele oncogeen allelen en getransformeerd via virale Cre-gemedieerde recombinatie, kunnen worden gebruikt om de pathogenese van astrocytomen in vitro en in vivo te modelleren door orthotopische injectie van getransformeerde cellen in hersenen van syngene, immuuncompetente nestgenoten.

Modeling Astrocytoom Pathogenese In Vitro En In Vivo Met behulp van corticale Astrocyten of neurale stamcellen uit Conditional, genetisch gemanipuleerde muizen

Celkweekmodellen gedetailleerde controle milieuomstandigheden en dus een krachtig platform om verschillende aspecten van neuronale cel biologie. We beschrijven een snelle, goedkope en betrouwbare wijze te isoleren, dissociëren en cultuur sensorische neuronen van kippenembryo's. Details van de ondergrond voorbereiding en immuuncytochemie zijn ook aanwezig.

Isolatie en Cultuur van Gedissocieerde sensorische neuronen van Chick Embryo

Ganglia (DRG) zijn structuren die de sensorische neuronen van het perifere zenuwstelsel. Wanneer gedissocieerd kunnen zij samen gekweekt met SC-achtige adipose afgeleide stamcellen (ASC), die een waardevol model voor in vitro zenuwregeneratie en myelinisatie bestuderen nabootsen van de in vivo omgeving bij de verwonding.

Achterwortelganglia Neuronen en Gedifferentieerde Adipose-afgeleide stamcellen: Een<em&gt; In Vitro</em&gt; Co-cultuur Model naar regeneratie van perifere zenuwen Bestudeer

Deze video-artikel toont het gebruik van de dorsale wortel ganglia (DRG)/kankercelmodel in adenocarcinoom van de ductus pancreaticus.

In Vitro Modellering van kanker zoals Neurale Invasion: De dorsale wortel ganglion Model

Perineural invasie is een agressief fenotype voorhoofd-en nek plaveiselcel cel carcinoom en andere tumoren. Het kuiken chorioallantoic membraan model is gebruikt voor het bestuderen van bloedvat, kanker invasie, en metastase. Hier laten we zien hoe dit model kan worden gebruikt om perineural invasie in vivo te beoordelen.

Het kuiken Chorioallantoic membraan in vivo model om Perineural invasie in hoofd en hals kanker te beoordelen

In het cocultuursysteem van dorsale wortelganglia en Schwann-cellen kan myelinisatie van het perifere zenuwstelsel worden bestudeerd. Dit model biedt experimentele mogelijkheden om perifere myelinisatie te observeren en te kwantificeren en om de effecten van interessante verbindingen op de myelineschede te bestuderen.

In vitro myelinisatie van perifere axonen in een cocultuur van dorsale wortel ganglionexplantaten en Schwann-cellen

We hebben een methodologie ontwikkeld om te beoordelen of neoplasmata van het zenuwstelsel in genetisch gemanipuleerde muizen de pathologie van hun menselijke tegenhangers nauwkeurig samenvatten. Hier passen we deze histologische technieken, gedefinieerde pathologische criteria en kweekmethodologieën toe op neurofibromen en kwaadaardige perifere zenuwschedetumoren die ontstaan in het P 0-GGFβ3 muismodel.

Identificeren, diagnosticeren en beoordelen van kwaadaardige perifere zenuwschedetumoren in genetisch gemanipuleerde muismodellen

Dit protocol biedt een techniek om geëxplanteerd dorsaal wortelganglion (DRG) van volwassen Sprague Dawley-ratten te oogsten en te kweken in een apparaat met meerdere compartimenten (MC).

Het oogsten, inbedden en cultiveren van dorsale wortelganglia in apparaten met meerdere compartimenten om perifere neuronale kenmerken te bestuderen

Leer hoe je 3D- en 2D-dorsale wortelgangliacultures opzet uit één muis om tumor-zenuwinteracties te bestuderen. Deze methode optimaliseert neurietgroei en kwantificeert neuro-geleide kankermigratie.

Opstellen van in-vitromodellen van dorsale wortelgangliacultuur: complementaire benaderingen voor het onderzoeken van overspraak tussen kanker en zenuw (Video) | JoVE Journal

Opstellen van in-vitromodellen van dorsale wortelgangliacultuur: complementaire benaderingen voor het onderzoeken van overspraak tussen kanker en zenuw

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