Nickel Resin
Nickel Resin - Related Videos
02:38
Anion Exchange Resin–Based Detection of Viruses from Bioaerosols
0 Views •
2026
Source: Schaeffer, J. W., Chandler, et al. Detection of Viruses from Bioaerosols Using Anion Exchange Resin. J. Vis. Exp. (2018).This video demonstrates the detection of viruses from bioaerosols using an anion exchange resin. A liquid impinger containing the resin is used to capture viruses from the air. The resin is collected and treated with a virus lysis buffer containing carrier RNA. The lysate is transferred to a microcentrifuge tube for viral RNA isolation, followed by qRT-PCR to amplify...
03:54
Nickel Affinity Chromatography-Based Protein Purification: A Technique to Purify Polyhistidine-Tagged Recombinant Proteins from Bacterial Cell Lysate
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2025
In this video, we demonstrate the nickel affinity chromatography technique to purify histidine-tagged pyrophosphokinase enzymes from Clostridium difficile bacteria.
07:51
Acrylic Resin Molding Based Head Fixation Technique in Rodents
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Cited by 4 •
2016
Traditional head fixation techniques have used metallic frames attached on the skull as an interface for head fixation apparatus. This protocol demonstrates a frameless, acrylic resin molding based head fixation technique for behavioral tasks in rodents.
08:40
Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules
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Cited by 3 •
2014
This article describes the preparation of well-ordered nickel nanofoams via electroless metal deposition onto nanoporous templates obtained from self-assembled diblock copolymer based supramolecules.
07:05
Clear Resin Casting of Arthropods for Use in Education, Outreach, and Research
0 Views •
2026
Here we show key steps in the process of creating high-quality, resin-embedded arthropods for educational instruction and outreach.
FAQs
Chelated nickel ions on the resin coordinate with histidine residues in a polyhistidine tag. This interaction gives tagged proteins a binding advantage over many other proteins in a complex sample. The selectivity depends on the tag-mediated contact between the protein and immobilized metal, allowing the target to be retained while untagged components remain available for removal.
Imidazole or an altered pH can disrupt the interaction between the polyhistidine tag and immobilized nickel, releasing the bound protein. These conditions therefore control the transition from retention to elution. Their role is especially important when the goal is to recover the target protein after contaminants have been removed during washing.
Washing removes contaminants that remain in the sample but do not bind as selectively as the polyhistidine-tagged target. This step improves the purity of the retained protein before elution. In biochemical work, effective washing helps produce a preparation more suitable for structural studies, enzyme assays, antibody research, or other experiments requiring purified biomolecules.
A typical workflow applies a protein-containing mixture, allows the polyhistidine-tagged target to interact with the immobilized nickel, washes away unwanted components, and then elutes the retained protein. Cell lysates are one supported starting material, although the same chromatographic principle can be applied to other complex mixtures containing the engineered target.
Nickel resin is useful when a recombinant protein has been engineered with a polyhistidine tag and must be separated from a complex mixture. Its use is relevant when downstream experiments require purified, functional biomolecules. Supported applications include preparing proteins for structural studies, enzyme assays, antibody research, and related biochemical investigations.
Purified proteins obtained with nickel resin can provide material for structural studies, enzyme assays, and antibody research. Removing contaminants is important because these experiments require purified, functional biomolecules rather than an unresolved mixture. The purification step therefore supports biochemical analysis by supplying a target preparation that can be examined in more focused experimental settings.