Aseptic Practices
Aseptic Practices - Related Videos
10:48
Aseptic Technique in Environmental Science
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner Aseptic technique is a fundamental skill widely practiced in the field of environmental microbiology that requires a balance of mindfulness and practice in the laboratory. Proper use of this technique reduces the likelihood of bacterial or fungal contamination of reagents, culture media, and environmental samples. Aseptic technique is also vital to ensure data integrity and...
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
09:46
Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening
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Cited by 9 •
2018
This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.
01:26
Good Manufacturing Practices
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2026
Good Manufacturing Practices (GMP) constitute a foundational set of guidelines that ensure the production of safe, consistent, and high-quality products, particularly in industries such as pharmaceuticals, biotechnology, and food processing. These protocols encompass all aspects of production, from the sourcing of raw materials to the final distribution of the finished product.A core pillar of GMP is stringent hygiene and sanitation across all production environments. This includes routine...
FAQs
Separation limits opportunities for contaminants to move from nonsterile areas, materials, or equipment into controlled work zones. This principle makes handling more deliberate by preserving clear boundaries between items intended to remain uncontaminated and those that may introduce microorganisms. In bioengineering workflows, maintaining these boundaries helps protect cultures, biomaterials, bioreactors, and biologic products.
Sterilization and disinfection provide distinct control measures within an aseptic workflow. Their use helps manage microorganisms associated with materials, equipment, and work environments before or during laboratory and manufacturing operations. Applying these measures consistently supports contamination control and helps prevent unwanted changes to experimental results, product quality, and process reliability.
Consistency reduces variation in how materials, equipment, and work areas are handled. When personnel repeatedly apply controlled handling, protective measures, and surface separation, contamination is less likely to introduce unexplained changes between experiments or production runs. This supports reproducibility, protects biologic products, and helps distinguish genuine biological effects from problems caused by inconsistent process conditions.
A coordinated workflow combines controlled handling with sterilization or disinfection, appropriate personal protective equipment, and deliberate separation of sterile and nonsterile surfaces. These elements work together rather than serving as isolated precautions. Their combined use helps maintain the condition of materials and equipment throughout laboratory or manufacturing operations and supports a more reliable contamination-control process.
They are especially important when work involves cell cultures, biomaterials, bioreactors, or biologic products, because contamination can alter results, compromise safety, or disrupt production. The same principles apply across tissue engineering, pharmaceutical development, and scalable manufacturing. In each setting, contamination control helps preserve material quality, experimental reliability, and consistency as processes become more complex.
Consistent application helps preserve contamination-free conditions needed for dependable laboratory and manufacturing operations. The resulting benefits include more reproducible experiments, protection of developing biologic products, and fewer disruptions to production. For bioengineering programs, this supports reliable work with cultures, biomaterials, and bioreactors while helping maintain product quality across research and development activities.