Intracellular Metabolite Profiling
Intracellular Metabolite Profiling - Related Videos
10:50
Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
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Cited by 5 •
2014
Plant biomass offers a renewable resource for multiple products, including fuel, feed, food, and a variety of materials. In this paper we investigate the properties of tobacco tree (Nicotiana glauca) and poplar as suitable sources for a biorefinery pipeline.
03:01
Sample Preparation for Metabolomics: A Method to Prepare Cell Samples for Metabolite Profiling
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2023
This video describes a technique of preparing breast cancer cell samples for metabolite profiling. Breast cancer cells are treated with estradiol, which binds to the estrogen receptor alpha and causes change in the metabolite...
09:16
FIBS-enabled Noninvasive Metabolic Profiling
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2014
A description of how to calibrate Förster Resonance Energy Transfer integrated biological sensors (FIBS) for in situ metabolic profiling is presented. The FIBS can be used to measure intracellular levels of metabolites noninvasively aiding in the development of metabolic models and high throughput screening of bioprocess conditions.
02:23
A Liquid Chromatography–Mass Spectrometry-Based Approach for Analyzing Metabolites from S. aureus
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2025
Source: Samuels, D. J., et al. A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus. J. Vis. Exp. (2017)This video demonstrates the use of liquid chromatography–mass spectrometry to identify and quantify intracellular metabolites from S. aureus. It outlines the steps involved in metabolite extraction, peptide normalization, liquid chromatography, and mass spectrometric analysis.
01:18
Detection of Human Fecal Metabolites Using a Bacterial Biosensor
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2026
Begin with a bacterial biosensor culture suspended in a medium.These engineered bacteria produce a transcription factor that, upon binding to a specific fecal metabolite, activates a promoter associated with a fluorescent reporter gene.To this culture, add a filtered human fecal sample containing metabolites, such as lactate, that are involved in host-microbiome interactions.Dilute this mixture with a fresh medium to maintain an optimal reaction volume.Incubate without shaking to mimic the...
FAQs
Rapid quenching is used to preserve metabolites at the moment of sampling, so the measured composition more closely represents the cell’s existing biochemical state. Cell extraction then releases those small molecules for analysis. This pairing matters because the resulting snapshot can be related to metabolic activity, pathway behavior, energy use, and product formation in engineered systems.
Liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy provide complementary analytical routes. The chromatography-based platforms separate metabolites before mass-based identification, whereas NMR spectroscopy offers a different measurement approach. Selecting among them determines how the sample’s small-molecule composition is resolved and identified for downstream metabolic interpretation.
By comparing metabolite profiles before and after a genetic or environmental change, researchers can associate altered small-molecule patterns with pathway activity. In bioengineering, that association helps explain how an intervention affects cellular metabolism rather than only whether a final product changes. The profiles therefore provide a biochemical link between system-level changes and observed energy use or product formation.
Intracellular metabolite profiling measures the cellular biochemical snapshot that can support metabolic flux analysis. The measured metabolite information helps researchers interpret pathway activity and assess how genetic or environmental changes influence metabolism. Keeping these roles distinct allows bioengineers to use concentration patterns as evidence when evaluating pathway behavior, energy use, and product formation.
The workflow begins with rapid quenching, followed by cell extraction to retain and release intracellular metabolites. The extract is then analyzed with liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, or nuclear magnetic resonance spectroscopy. Data from these measurements are interpreted as a biochemical profile, which can then be connected to pathway activity, energy use, or product formation.
Bioengineers can apply the approach when they need to evaluate how genetic or environmental changes influence an engineered cell. The resulting measurements can guide strain optimization, reveal changes associated with pathway activity, and support bioprocess monitoring. This makes the method useful across efforts to improve cellular production systems rather than relying only on a final product measurement.
Metabolite profiles can connect pathway activity and energy use with product formation, giving engineers evidence for refining an engineered cell. In this context, the data support designs aimed at producing pharmaceuticals, biofuels, and other bioproducts. They also help relate strain-level changes to bioprocess behavior, which is important when moving from cellular engineering to production-focused evaluation.