Phloem-rich Tissue

Phloem-rich tissue is plant tissue enriched in phloem, the vascular network that distributes sugars, signaling molecules, and other organic nutrients from source organs to growing or storage tissues. Its transport system relies on sieve-tube elements linked by sieve plates and supported by companion cells; loading solutes raises osmotic pressure, draws in water, and drives bulk flow toward sinks through pressure differences. In bioengineering, phloem-rich tissue provides a model for studying biological fluid transport, plant growth, and source-to-sink regulation. Its organization may also inform engineered plant systems and bioinspired networks designed for efficient solute distribution.

Phloem-rich Tissue - Related Videos

Education

JoVE Core - Biology

Phloem and Sugar Transport

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2026

Like many living organisms, plants have tissues that specialize in specific plant functions. For example, shoots are well adapted to rapid growth, while roots are structured to acquire resources efficiently. However, sugar production is primarily restricted to the photosynthetic tissues that reside in the leaves of angiosperm plants. Sugar and other resources are transported from photosynthetic cells to other specialized tissues by a process called translocation.Within a plant, tissues that...

Research

JoVE Journal - Biology
Free Sample

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method

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Cited by 88 •

2013

Knowledge of the composition of the phloem sap as well as the mechanism of its loading and long-distance transport is essential for the understanding of long-distance signaling in plant development and stress/pathogen response and of assimilate transport. This manuscript describes the collection of phloem exudates using the EDTA-facilitated method.

Research

JoVE Journal - Biology
Free Sample

Phloem Sap Sampling from Brassica napus for 3D-PAGE of Protein and Ribonucleoprotein Complexes

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Cited by 13 •

2018

Here we present a protocol to analyze the protein composition of large native protein:protein and protein:nucleic acid complexes from oilseed rape (B. napus) phloem exudate using a 3D polyacrylamide gel electrophoresis (PAGE) approach combining blue native (BN) with two denaturing PAGEs followed by mass spectrometric identification.

A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements

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Cited by 24 •

2015

Electrical Penetration Graph (EPG) is a well-established technique for studying the feeding behavior of stylet-bearing insects. Here we show a new application of EPG as a non-invasive tool for the acquisition of intracellular electrophysiology recordings of sieve elements (SEs), the cells that form the phloem vasculature in plants.

Working with Human Tissues for Translational Cancer Research

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Cited by 4 •

2015

Translational cancer research is dependent on extraction of human tissues. Much work has gone into optimizing extraction methods for ex vivo analysis. Here, we describe tissue processing methods allowing for maximal data output from limited samples.

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