Air Pouch Assay
Air Pouch Assay - Related Videos
03:41
An Air Pouch Mouse Model for Lipopolysaccharide-Induced Inflammatory Exudate Collection
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2025
This video describes the procedure for generating an air pouch in the subcutaneous tissue of mice and collecting inflammatory exudates in response to lipopolysaccharide administration in vivo. This video also covers the analysis of the neutrophil infiltration in the air pouch.
03:47
Developing an Experimental Aneurysm Model in a Rabbit Using an Elastase-Treated Vessel Pouch
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2025
Source: Wanderer, S., et al. Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit. J. Vis. Exp. (2020)This video demonstrates the creation of an experimental aneurysm model in a rabbit using an elastase-treated vessel pouch. After excising a segment of the right CCA, the pouch is treated with elastase, then sutured to the left CCA to restore blood flow. The resulting stress on the weakened pouch walls induces aneurysm formation.
07:34
Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects
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Cited by 11 •
2011
An optimized technique for the microsurgical creation of arterial bifurcation aneurysms mimicking bifurcation human cerebral aneurysms is described. A venous pouch is sutured into an artificially created true bifurcation of both common carotid arteries. Facilitated microsurgical techniques and aggressive postoperative anticoagulation and analgesia lead to minimized morbidity rates and high aneurysm patency rates.
06:28
Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
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Cited by 21 •
2018
This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow.
06:11
Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit
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Cited by 11 •
2020
Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits.
FAQs
Reinflation helps maintain the pouch’s temporary structure after the initial air injection. Preserving this accessible cavity supports consistent recovery of inflammatory exudate and pouch-lining tissue after stimulation. Maintaining the site is therefore important when researchers want to compare leukocyte recruitment, cytokine levels, or other inflammatory mediators across experimental conditions.
The irritant or inflammatory stimulus initiates the localized response that accumulates measurable exudate and changes the pouch lining. Those collected materials provide biological evidence of the response, including recruited leukocytes and soluble mediators such as cytokines. The selected stimulus therefore determines which inflammatory changes can be examined in the assay.
This model supports analysis of several linked features of acute inflammation rather than a single endpoint. Researchers can examine leukocyte recruitment, cytokine production, other inflammatory mediators, and changes in the pouch-lining tissue. Considering these readouts together helps connect cellular movement with molecular signaling at the same localized site.
The workflow begins by creating the subcutaneous cavity with sterile air. Researchers may reinflate it to maintain the pouch, then introduce an irritant or other inflammatory stimulus. After the response develops, they collect the exudate and pouch-lining tissue for analysis, allowing the same experimental site to provide cellular and molecular readouts.
Two principal sample types are available: inflammatory exudate from the cavity and tissue from the pouch lining. Exudate can be examined for recruited leukocytes, cytokines, and other mediators, while the lining provides tissue-level information about the local response. Together, these samples support complementary assessment of inflammation within the model.
The assay is useful when a study needs a localized in vivo inflammatory response with an accessible sampling site. Treatment effects can be assessed by comparing inflammatory readouts under controlled experimental conditions, including leukocyte recruitment, cytokines, other mediators, and pouch-lining responses. This makes the model relevant to compound evaluation and acute-inflammation research in biology.