Bal Fluid Collection
Bal Fluid Collection - Related Videos
11:47
Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield
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Cited by 47 •
2014
We describe a research technique for fiberoptic bronchoscopy and bronchoalveolar lavage using low pressure suction. The technique is used to harvest immune cells from the lung bronchoalveolar surfaces. Local anesthetic and mild conscious sedation (midazolam) is used. Subjects tolerate the procedure well and experience minimal side effects.
08:08
A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
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Cited by 271 •
2008
Transgenic (Tg) mouse models of AD provide an excellent opportunity to investigate how and why Aβ or tau levels in CSF change as the disease progresses in human patients. Here, we demonstrate a refined cisterna magna puncture technique for serial CSF sampling from the mouse.
02:17
Bronchoalveolar Lavage Fluid Collection from a Mouse Model Infected with Non-typeable Haemophilus influenzae
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2025
Source: Venuprasad, K., et al. Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation. J. Vis. Exp. (2016)This video demonstrates the collection and processing of bronchoalveolar lavage fluid from a euthanized mouse infected with non-typeable Haemophilus influenzae for downstream analysis.
08:50
Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma
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2025
In vivo, intercellular communications underpinning many central nervous system diseases, such as glioblastoma (GBM), are notoriously difficult to measure and characterize. Here, we describe procedures of cerebral open-flow microperfusion (cOFM) that can be employed to sample interstitial fluid components in a longitudinal animal model of GBM.
06:40
An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
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Cited by 80 •
2018
This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal cord.
FAQs
Sterile saline enters the chosen airway through a bronchoscope and mixes with material present along the lower respiratory tract surface. Aspiration then retrieves this fluid for analysis, including airway-lining liquid, immune cells, microorganisms, proteins, and other cellular components. Selecting a specific segment helps associate the recovered biological material with a defined region of the lung.
A recovered sample can contain airway-lining liquid, immune cells, microorganisms, proteins, and other cellular components. Together, these materials provide complementary information: cellular contents can indicate immune activity, microorganisms can support investigation of infection, and proteins or lining-fluid components can contribute to molecular assessments of inflammation or tissue injury.
BAL fluid collection provides material directly associated with the lower respiratory tract, allowing investigators to examine biological changes linked to inflammation, infection, immune responses, and tissue injury. Researchers can compare cellular or molecular profiles between experimental conditions, helping connect changes in the sampled airway environment with lung function or disease mechanisms.
Differences in recovered cellular or molecular profiles can reflect changes in pulmonary inflammation, infection, immune responses, or tissue injury. Comparing samples across conditions allows researchers to identify biological patterns associated with respiratory disorders or experimental treatments. The value lies in interpreting the collection as a source of measurable airway information rather than as an isolated fluid sample.
The workflow begins by using a bronchoscope to access a selected airway segment. Sterile saline is delivered into that region and then aspirated to recover material from the lower respiratory tract. The collected fluid can subsequently be examined for immune cells, microorganisms, proteins, airway-lining liquid, and other cellular components relevant to the study.
Researchers may choose this approach when they need lower-respiratory-tract material to investigate pulmonary inflammation, infection, immune responses, tissue injury, or lung function. It is also useful for comparing cellular and molecular profiles during studies of respiratory disorders and experimental treatments, while providing a minimally invasive way to examine biological processes in the lung.
Analysis can reveal cellular and molecular patterns associated with disease mechanisms in the lung. Investigators may assess immune-cell content, microorganisms, proteins, airway-lining material, and other cellular components, then compare these findings across samples. Such comparisons can help characterize inflammation or infection and evaluate biological responses connected with respiratory disorders or experimental treatments.