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An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
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1Shanghai Advanced Research Institute,University of Chinese Academy of Sciences, Chinese Academy of Science, 2Shanghai Institute of Materia Medica,University of Chinese Academy of Sciences, Chinese Academy of Science, 3Center of Functionally Integrative Neuroscience, Department of Clinical Medicine,Aarhus University, 4Sino-Danish Center for Education and Research (SDC), 5Department of Neurology,Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine, 6Department of Nuclear Medicine and PET-centre,Aarhus University Hospital
Chapters
- 00:05Title
- 00:42Preparation for CSF Collection
- 01:57Surgery to Expose the Cisterna Magna
- 03:34CSF Collection
- 05:53Results: Aβ ELISA Analysis of CSF Sample Collected
- 06:13Conclusion
This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal cord.
