Bioluminescent Bacteria

Bioluminescent bacteria are microorganisms that produce and emit visible light through a regulated biochemical reaction, making them important models for studying microbial physiology, symbiosis, and environmental signaling. In many species, the enzyme luciferase oxidizes the substrate luciferin in the presence of oxygen, releasing energy as blue-green light; population density can control light production through quorum sensing and autoinducer signaling. Researchers use these organisms as biosensors, reporter systems, and tools for investigating gene regulation, host-microbe interactions, marine ecosystems, and environmental monitoring, while their light-producing pathways support biotechnology and ecological research.

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JoVE EoE - Bacterial Growth and Techniques

Expression of the Luminescence Operon in Marine Bioluminescent Bacteria

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2025

Source: Brodl, E., et al. In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria. J. Vis. Exp. 1 (2018)This video demonstrates the cultivation of marine bioluminescent bacteria to observe quorum-sensing–mediated activation of the luminescence operon, enabling real-time monitoring of gene expression and light emission for studies in microbial communication and gene regulation.

Modeling Infection in Neonatal Mice Using Encapsulated Bioluminescent Bacteria

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2026

Source: Seman, B. G., et al. A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis. J. Vis. Exp. (2020)The video demonstrates a neonatal mouse model for studying systemic infection using encapsulated bioluminescent bacteria. Neonatal pups are injected with high- or low-dose bacterial suspensions and returned to their mother for postnatal care. The encapsulated bacteria evade early immune detection, spread systemically, and are visualized over time using luminescence imaging. This model...

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

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Cited by 18 •

2014

Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria.

Bioluminescent Bacterial Imaging In Vivo

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Cited by 9 •

2012

This article describes the administration of lux-tagged bacteria to mice and subsequent in vivo analysis using IVIS bioluminescence imaging.

Bioluminescence Imaging of Bacterial Infections in Mice

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2026

Source: de Vries, C. R., et al., A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection. J. Vis. Exp. (2020).This video demonstrates a bioluminescent imaging technique to monitor bacterial infection progression in a live mouse model. Pathogenic bacteria engineered with the luxABCDE reporter system emit light at the infection site, allowing visualization of metabolically active bacteria. By imaging the mouse over time, researchers can non-invasively track infection...

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