Epidermal Repair
Epidermal Repair - Related Videos
03:21
Epidermal Laser Wounding: Inducing Localized Damage to Examine Repair Processes in C. elegans
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2023
This video introduces a method of laser wounding, which is used to cause very precise injuries in the worm epidermis. The sample protocol is from a study of wound response and repair.
21:16
An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
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Cited by 57 •
2009
In this video, we demonstrate the EpiDerm Skin Irritation test (EpiDerm SIT) developed and validated for in vitro skin irritation testing of chemicals, including cosmetic and pharmaceutical ingredients.
02:25
Overview of DNA Repair
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2020
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage. Chemically...
11:12
Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.
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Cited by 10 •
2013
The embryonic epidermis of very late stage Drosophila embryos provides an in vivo system for rapid puncture wound response analysis and can be combined with genetic manipulations or chemical microinjection treatments to advance studies in wound healing for translation into mammalian models.
01:54
Base Excision Repair
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2020
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template. The first step of...
FAQs
Migration by keratinocytes at the wound edge provides the first cellular response that covers the damaged area. These cells move across the wound surface before the replacement tissue is fully rebuilt. This early movement is important because it starts re-epithelialization, the process that reconnects the epidermal surface and supports restoration of the skin barrier.
After keratinocytes migrate, proliferation increases the number of cells available to replace those lost through injury. Differentiation then organizes these cells into a stratified epidermis, meaning one arranged in multiple layers. Together, these processes produce more than temporary coverage: they rebuild the layered architecture required for effective barrier restoration.
Inflammation and extracellular matrix remodeling provide coordinated support for the cellular events that rebuild the epidermis. The extracellular matrix is the surrounding structural network that cells interact with, while inflammation is part of the injury response. Their coordination with keratinocyte migration, proliferation, and differentiation helps repair proceed as an integrated biological process.
Persistent or impaired re-epithelialization indicates that the epidermis is not restoring its surface effectively after injury. Studying this problem helps connect disrupted repair with disorders involving the skin’s outer layer. It also directs attention to which stages may be affected, including keratinocyte movement, cell replacement, layered rebuilding, inflammation, or extracellular matrix remodeling.
A useful study framework follows the sequence of keratinocyte migration, proliferation, and differentiation, while also considering inflammation and extracellular matrix remodeling. Researchers can then relate these coordinated events to the final outcome of barrier restoration. This approach supports comparisons between normal healing and situations in which re-epithelialization remains persistent or incomplete.
Knowledge of Epidermal Repair supports research on skin disease, engineered tissues, biomaterials, and therapies intended to improve barrier restoration. In each area, the central goal is to understand or support the biological events that rebuild the epidermis. This makes the topic relevant both to normal wound-healing research and to efforts addressing defective re-epithelialization.