Estrogen Receptor Alpha
Estrogen Receptor Alpha - Related Videos
03:52
Estrogen Receptor-Reporter Activity Assay to Screen Phytoestrogen Estrogenic Activity
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2025
This video describes a cell-based reporter assay to determine the estrogenic activity of plant-derived compounds. A compound with estrogen-like activity mimics its binding to estrogen receptors and drives downstream reporter enzyme gene transcription. The reporter enzyme generates bioluminescence in a reaction, indicating the estrogenic activity of the compound.
07:42
Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope
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2011
The goal of this article is to demonstrate a method to optimize immunofluorescent detection of estrogen receptor-α (ERα) in rat pial arterial slices using a digital immunofluorescent microscope.
16:24
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
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Cited by 9 •
2014
Molecular signaling through both estrogen and microRNAs are critical in breast cancer development and growth. Estrogen activates the estrogen receptors, which are transcription factors. Many transcription factors can regulate the expression of microRNAs, and estrogen-regulated microRNAs can be profiled using different large-scale techniques.
09:07
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
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Cited by 5 •
2018
We present a method for analyzing the 4-hydroxy-tamoxifen-dependent estrogen receptor alpha ligand-binding domain dimerization activity using the mammalian two-hybrid assay.
10:36
Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
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Cited by 5 •
2016
Integration of data from genome-wide sequencing experiments and metabolomics experiments is a challenge. In this paper we report, for the first time, generation, analysis and integration of transcriptome, cistrome and metabolome data from breast cancer cells treated with estradiol.
FAQs
Estrogen binding triggers a conformational change in ERα, altering the receptor’s functional state. The activated receptor can then form dimers, meaning paired receptor complexes, and engage estrogen response elements in DNA. These events connect hormone recognition with regulation of target-gene expression, providing a molecular explanation for how estrogen signals can produce tissue-specific biological effects.
ERα can regulate genes through two related routes: direct association with estrogen response elements in DNA and interaction with other transcriptional regulators. This distinction matters because gene control does not depend on a single type of molecular contact. Studying both routes helps researchers interpret how estrogen-responsive pathways influence tissue function and hormone-dependent biology.
ERα provides a framework for examining how estrogen-responsive signaling relates to distinct biological settings. In reproductive biology and development, it helps researchers investigate hormone-dependent processes; in tissues such as breast, uterus, and bone, it supports analysis of tissue function. Comparing these contexts can reveal where estrogen signaling is relevant without assuming that the same response occurs in every tissue.
These assays address two complementary questions. Expression measurements examine the receptor’s presence or level, whereas activity measurements examine its functional participation in estrogen-responsive signaling. Together, they support investigation of whether ERα is available as a molecular component and whether its pathway is active. This distinction is useful when interpreting endocrine signaling and hormone-dependent tissue biology.
Within breast cancer biology, ERα assays help researchers measure receptor expression or activity while examining estrogen-responsive pathways. The resulting information can support studies of how hormone-dependent signaling relates to cancer biology and can support investigation of therapies designed to block or modify those pathways. The assays therefore connect molecular receptor analysis with disease-focused research.
Research on ERα can address how estrogen-linked regulation participates in reproductive biology, development, and tissue function. Its relevance extends across breast, uterus, and bone, making it useful for connecting molecular endocrine signaling with tissue-level questions. This breadth also explains why ERα studies appear in both basic biology and disease-focused research, including breast cancer investigations.