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Chapters
- 00:05Title
- 01:31Cell Preparation and Treatment
- 04:19RNA Extraction
- 06:40miRNA Profiling Analysis
- 08:17Confirmation of miRNA Regulations using Separate qPCR Analysis
- 10:49Results: Estrogen Regulates miRNA in Breast Cancer Cells
- 15:36Conclusion
Molecular signaling through both estrogen and microRNAs are critical in breast cancer development and growth. Estrogen activates the estrogen receptors, which are transcription factors. Many transcription factors can regulate the expression of microRNAs, and estrogen-regulated microRNAs can be profiled using different large-scale techniques.
