Follicular Recruitment

Follicular recruitment is the process by which a group of ovarian follicles begins coordinated growth, making it a key stage in reproductive biology and fertility. During each reproductive cycle, rising follicle-stimulating hormone (FSH) supports the transition of selected follicles into actively growing antral follicles; as FSH levels decline, the follicle with the greatest sensitivity often gains dominance while others undergo atresia. Studying follicular recruitment helps explain ovarian function, follicle selection, ovulation, and menstrual-cycle regulation. It also informs research on infertility, assisted reproductive technologies, ovarian aging, and conditions involving abnormal follicle development.

Follicular Recruitment - Related Videos

Research

JoVE Journal - Cancer Research

Lung Tumor Cell Recruitment Assay

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2019

This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis.

An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment

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2025

The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

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Cited by 28 •

2015

The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.

Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization

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2025

This protocol outlines standardized methods to isolate and process follicular fluid, somatic cells, and immature oocytes from IVF procedures, enabling high-quality molecular and cellular analyses. These approaches support translational research into fertility, reproductive aging, and ovarian dysfunction using materials typically discarded in clinical care.

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

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Cited by 5 •

2016

Optimized procedures for the isolation of single follicles, cytoplasmic RNA microinjections, the removal of surrounding cell layers, and protein expression in Xenopus oocytes are described. In addition, a simple method for fast solution changes in electrophysiological experiments with ligand-gated ion channels is presented.

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