Intestinal Villi

Intestinal villi are fingerlike projections lining the small intestine that greatly increase its internal surface area for nutrient absorption. Each villus is covered by epithelial cells, whose microvilli form a brush border, and contains blood capillaries and a lymphatic vessel called a lacteal. As digestion breaks food into smaller molecules, sugars and amino acids pass through the epithelium into blood, while many dietary lipids enter the lacteal and lymphatic system. Their specialized structure illustrates how anatomy supports physiological function and provides a foundation for studying digestion, nutrient transport, intestinal disease, and malabsorption.

Intestinal Villi - Related Videos

Research

JoVE Journal - Bioengineering

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

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Cited by 68 •

2016

We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

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Cited by 2 •

2021

The procedure describes isolation of the villi from the mouse intestinal epithelium undergoing dedifferentiation to determine their organoid forming potential.

Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model

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2023

In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.

A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

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Cited by 81 •

2015

In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

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Cited by 166 •

2016

Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues.

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