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3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
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3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

DOI:
10.3791/61809-v

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06:40 min

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April 01, 2021

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, , , , , ,
1Department of Biology and Chemical Biology,Stevens Institute of Technology, 2Department of Pharmacology,Pennsylvania State University College of Medicine

Chapters

  • 00:04Introduction
  • 00:54Mice
  • 01:27Duodenum Isolation and Preparation
  • 01:54Villi Isolation by Scraping
  • 03:32Plating of Villi on BME-R1 Matrix
  • 05:02Results: Organoid Formation from the Dedifferentiating Villi
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

The procedure describes isolation of the villi from the mouse intestinal epithelium undergoing dedifferentiation to determine their organoid forming potential.

Tags

Keywords: 3D CulturingOrganoidsIntestinal Villi EpitheliumDe-differentiationStem Cell MarkersScrapingMesenchymeNiche SignalsSmad4 KnockoutBeta-catenin Gain Of FunctionTamoxifenDuodenumPBSMesh StrainerGlass SlidesMucusCrypts

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