Low Phototoxicity
Low Phototoxicity - Related Videos
05:08
In Vitro Phototoxicity Assay: A PDT-based Method to Evaluate the Phototoxic Potential of a Photosensitizer in Lung Cancer Cells
0 Views •
2023
Photodynamic therapy (PDT) is a non-invasive and non-surgical method for lung cancer treatment. Photosensitizers selectively accumulate in tumor tissue and lead to tumor cell death in the presence of oxygen and the proper wavelength of light. In this protocol video, PDT-based method is used to evaluate the phototoxic potential of a potential photosensitizer in lung cancer cells.
03:59
In Vivo Antitumor Efficacy Analysis of PDT: A Technique to Determine the Phototoxic Potential of a Photosensitizer in Tumor Bearing Mice
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2023
Photodynamic therapy (PDT) is a non-invasive and non-surgical method for lung cancer treatment. Photosensitizers selectively accumulate in tumor tissue and lead to tumor cell death in the presence of oxygen and the proper wavelength of light. This video describes the in vivo anti-tumor efficacy analysis using photosensitizer containing nanoparticles in a tumor-bearing mouse, to determine the phototoxic potential of the photosensitizer.
07:49
Rod-based Fabrication of Customizable Soft Robotic Pneumatic Gripper Devices for Delicate Tissue Manipulation
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Cited by 10 •
2016
This protocol describes a rod-based approach, combining 3D-printing and soft lithography techniques for fabricating the soft gripper devices. This approach eliminates the need for an external air source by incorporating a chamber component and reduces the chance of occlusion during the sealing process, particularly for miniaturized pneumatic channels.
10:09
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
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Cited by 11 •
2015
We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.
07:54
Cholesterol Efflux Assay
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Cited by 74 •
2012
The cholesterol assay is designed to quantitate the rate of cholesterol efflux from cultured cells and the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of labelling cells with cholesterol, equilibration of cholesterol among intracellular pools and release of cholesterol to an extracellular acceptor.
FAQs
Lower excitation intensity reduces the light-driven reactions that can generate reactive oxygen species, which are associated with cellular stress. This adjustment can help preserve cell viability and normal behavior during imaging. Researchers can therefore collect fluorescence data while reducing the likelihood that illumination itself will alter the biological process under observation.
Shorter exposure times limit the duration of illumination delivered to the specimen, reducing cumulative light stress. They also help slow photobleaching, the loss of fluorescence signal during repeated or continued excitation. Optimizing exposure time is especially important when images must be acquired across many time points or when quantitative measurements depend on a stable signal.
Suitable wavelengths and fluorophores can support useful fluorescence signals while helping reduce biological damage from illumination. Their selection is therefore part of the experimental design rather than a purely imaging-related choice. Matching these variables to the specimen and measurement goal can help maintain image quality while limiting cellular stress and preserving normal behavior.
Repeated illumination can increase the cumulative light burden on cells, particularly during time-lapse imaging. Limiting unnecessary imaging cycles reduces exposure and can help preserve viability, behavior, and fluorescence signal over the experiment. This consideration is central when tracking dynamic cellular processes whose interpretation depends on observing cells without substantially disturbing them.
Begin by reducing excitation intensity and exposure time, then select wavelengths and fluorophores that provide a usable signal under those conditions. Limit repeated illumination to the time points needed for the biological question. This balance helps retain informative images while reducing reactive oxygen species formation, cellular stress, photobleaching, and loss of experimental reliability.
It is particularly valuable for live-cell imaging, developmental biology, long-term time-lapse studies, and quantitative analysis of dynamic cellular processes. In these settings, illumination can influence viability or behavior and thereby confound interpretation. Reducing light-related damage helps researchers follow biological changes over time while preserving the condition and function of the observed cells.