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Chapters
- 00:05Title
- 01:32Preparation of 3H-cholesterol
- 01:53Label Cellular Cholesterol
- 02:37Equilibrium Incubation
- 03:19Cholesterol Efflux Incubation
- 04:10Processing Samples
- 05:45Data Analysis
- 06:11Representative Results: Assaying Modulators of Cholesterol Efflux in Macrophages
- 07:32Conclusion
The cholesterol assay is designed to quantitate the rate of cholesterol efflux from cultured cells and the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of labelling cells with cholesterol, equilibration of cholesterol among intracellular pools and release of cholesterol to an extracellular acceptor.
Tags
Cholesterol Efflux AssayCholesterol ContentCellular MembranesApoptosisNecrosisIntracellular SynthesisPlasma LipoproteinsCholesterol SynthesisCholesterol UptakeDeficiencies Of CholesterolExcessive CholesterolHepatocytesAdrenocortical CellsCholesteryl EstersATP Binding Cassette Transporter Proteins A1 (ABCA1)ATP Binding Cassette Transporter Proteins G1 (ABCG1)Scavenger Receptor Type B1High Density Lipoprotein (HDL)Apolipoprotein A-I
