Pulmonary Endothelial Cells
Pulmonary Endothelial Cells - Related Videos
10:46
Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
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Cited by 89 •
2010
Here, we describe a protocol for isolation and culture of murine pulmonary endothelial cells. This method comprises mechanic and enzymatic lung tissue dissociation as well as a 2-step purification process using anti-PECAM-1 and anti-ICAM-2 antibodies conjugated to magnetic beads, which produces a pure endothelial cell population of mostly microvascular origin.
03:14
Obtaining Cytotoxic Amyloids Following Bacterial Infection of Rat Pulmonary Endothelial Cells
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2025
Source: Balczon, R., et al. Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa. J. Vis. Exp. (2018)This video demonstrates the procedure for generating cytotoxic supernatant from pulmonary endothelial cells following infection with Pseudomonas aeruginosa. It outlines how bacterial effector proteins disrupt host cell structure and induce the release of cytotoxic amyloid oligomers into the supernatant.
08:55
In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
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Cited by 17 •
2013
The endothelial glycocalyx/endothelial surface layer is ideally studied using intravital microscopy. Intravital microscopy is technically challenging in a moving organ such as the lung. We demonstrate how simultaneous brightfield and fluorescent microscopy may be used to estimate endothelial surface layer thickness in a freely-moving in vivo mouse lung.
18:30
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
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Cited by 12 •
2013
This protocol presents a complete and detailed procedure to apply RNA-seq, a powerful next-generation DNA sequencing technology, to profile transcriptomes in human pulmonary microvascular endothelial cells with or without thrombin treatment. This protocol is generalizable to various cells or tissues affected by different reagents or disease states.
11:04
Isolation of Valvular Endothelial Cells
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Cited by 82 •
2010
We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.
FAQs
Cell junctions act as a selectively regulated barrier rather than an unrestricted seal. Their organization influences how readily solutes and other substances move between circulating blood and respiratory tissues, while associated signaling pathways adjust barrier behavior in response to changing conditions. Studying these controls helps explain how pulmonary vascular integrity is maintained or disrupted during inflammation and acute lung injury.
Mechanical and chemical cues can alter endothelial signaling, which in turn affects barrier function, blood flow, and interactions with circulating cells. These responses provide a way to examine how lung vessels adapt to their environment and how abnormal signals may contribute to vascular dysfunction. Experimental models use such cues to investigate disease mechanisms and evaluate potential drug effects.
Signaling pathways in the pulmonary endothelium help coordinate interactions between the vessel lining and the blood. By regulating leukocyte trafficking, they influence how immune cells move through or interact with the vascular interface. Their relevance to thrombosis reflects the broader role of endothelial responses in vascular biology, making these pathways useful targets for studying inflammation and clot-related disease processes.
Culture models allow researchers to examine pulmonary endothelial responses under controlled experimental conditions, while tissue models provide context within lung structures and vascular relationships. Together, these approaches support investigation of barrier behavior, signaling, vascular development, inflammation, and responses to chemical or mechanical cues. Comparing findings across models can connect cellular mechanisms with tissue-level changes relevant to disease.
They are examined in research on pulmonary hypertension, acute lung injury, inflammation, thrombosis, and vascular development. These settings make it possible to connect altered endothelial behavior with changes in pulmonary circulation and respiratory tissues. The resulting observations can clarify how disease-related signals affect vascular regulation and can help researchers assess mechanisms underlying pulmonary and respiratory disorders.
Experiments can reveal how the pulmonary vascular barrier responds to drugs and how endothelial signaling changes under disease-relevant conditions. Such findings support evaluation of compounds that influence permeability, inflammation, blood flow, or other vascular behaviors. They also provide biological context for developing therapies aimed at vascular and respiratory disorders, including conditions associated with pulmonary hypertension or acute lung injury.