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JoVE Journal
Biology

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Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
 

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

Article DOI: 10.3791/2316
December 14th, 2010

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Summary December 14th, 2010

Here, we describe a protocol for isolation and culture of murine pulmonary endothelial cells. This method comprises mechanic and enzymatic lung tissue dissociation as well as a 2-step purification process using anti-PECAM-1 and anti-ICAM-2 antibodies conjugated to magnetic beads, which produces a pure endothelial cell population of mostly microvascular origin.

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