Yersinia Ruckeri
Yersinia Ruckeri - Related Videos
10:33
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Cited by 5 •
2019
The Multi-Locus Variable-number tandem-repeat Analysis (MLVA) assay presented here enables inexpensive, robust and portable high-resolution genotyping of the fish-pathogenic bacterium Yersinia ruckeri. Starting from pure cultures, the assay employs multiplex PCR and capillary electrophoresis to produce ten-loci MLVA profiles for downstream applications.
01:30
Improved Differential Isolation of Yersinia Species Using a Modified Cefsulodin-Irgasan-Novobiocin Agar
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2025
Begin with cultures of Gram-negative Yersinia species and non-Yersinia Enterobacteriaceae.Using sterile needle inoculators, dot the cultures onto both traditional Cefsulodin-Irgasan-Novobiocin or CIN agar and modified CIN agar.Incubate the plates to promote bacterial growth.Both traditional and modified CIN agars support the growth of Yersinia and non-Yersinia Enterobacteriaceae that ferment mannitol to produce acidic byproducts.Acid production lowers the pH and triggers a color change,...
12:23
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
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Cited by 5 •
2015
Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.
02:14
Thermal Lysis-Based Genomic DNA Extraction from a Pathogenic Bacterium
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2025
Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This protocol extracts genomic DNA from Yersinia ruckeri by suspending isolated colonies in ultrapure water and applying heat to lyse the cells. Centrifugation separates the DNA-containing supernatant from cell debris, yielding template DNA suitable for PCR-based genotyping and epidemiological analysis.
08:36
Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth
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Cited by 2 •
2024
Bacteria colonize host tissues that vary in oxygen and iron bioavailability, yet most approaches to studying bacteria use aerated, rich media. This protocol describes culturing the human pathogen Yersinia pseudotuberculosis under varying iron concentrations and oxygen tension, and quantifying activity of the Yersinia type III secretion system, which is an important virulence factor.
FAQs
Disease progression begins when fish encounter the bacterium through water or contaminated material. Yersinia ruckeri can then colonize mucosal tissues, which form an early interface between the fish and its environment. From these tissues, it may enter the bloodstream and produce a systemic infection, linking the initial exposure route to inflammation, hemorrhage, and broader disease effects.
Mucosal tissues are important because they represent a colonization site after exposure. Their involvement provides a biological connection between environmental contact and later bloodstream invasion. Studying this stage helps explain how infection becomes systemic and can guide research into transmission, virulence, and interventions intended to reduce disease development in cultured fish.
These findings reflect different consequences of infection rather than isolated external changes. Inflammation indicates an activated tissue response, while hemorrhage signals damage associated with disease progression. Reddening around the mouth is a characteristic visible sign that can support recognition of enteric redmouth disease, especially when considered alongside evidence of systemic infection and observed mortality.
Diagnosis helps identify infections in affected fish, while outbreak surveillance extends observation across groups, facilities, or production settings. Together, they support recognition of disease occurrence and provide information for responding to transmission and mortality. These activities are important because monitoring can connect clinical signs with broader aquaculture biosecurity and husbandry decisions.
Research on Yersinia ruckeri biology and virulence provides information that can support vaccine development. Understanding how the bacterium reaches mucosal tissues, enters the bloodstream, and produces systemic effects helps frame the biological events that preventive strategies must address. Vaccination research therefore fits within a broader effort to reduce fish mortality and improve aquaculture production.
Because exposure can occur through water or contaminated material, biosecurity and husbandry are relevant tools for reducing opportunities for transmission and limiting outbreak consequences. Their value extends beyond an individual fish: coordinated management can support disease prevention, surveillance, and mortality reduction across aquaculture operations, contributing to more sustainable production of freshwater and marine fish.