Cell Cultivation Protocol
Cell Cultivation Protocol - Related Videos
09:01
Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory
0 Views •
Cited by 4 •
2016
We present a simple method to construct 3D nematode cultivation systems called NGT-3D and NGB-3D. These can be used to study nematode fitness and behaviors in habitats that are more similar to natural Caenorhabditis elegans habitats than the standard 2D laboratory C. elegans culture plates.
12:08
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
0 Views •
Cited by 5 •
2016
This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).
08:13
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
0 Views •
Cited by 8 •
2015
A novel reactor design, coined a high density bioreactor (HDBR), is presented for the cultivation and study of high density microbial communities. Here, the HDBR is successfully applied in a photobioreactor (PBR) configuration for the study of nitrogen metabolism by a mixed high density algal community.
11:01
Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
0 Views •
Cited by 23 •
2012
Here we describe the use of a self-assembling 3-dimensional scaffold to culture human neural progenitor cells. We present a protocol to release the cells from the scaffolds to be analysed subsequently e.g. by flow cytometry. This protocol might be adapted to other cell types to perform detailed mechanistically studies.
11:42
Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
0 Views •
Cited by 4 •
2014
Using a pneumatic bioreactor, we demonstrate the assembly, operation, and performance of this single-use bioreactor system for the growth of mammalian cells.
FAQs
These conditions create the controlled environment required for cells to remain viable and biologically useful. Defined culture media supplies a consistent growth setting, while appropriate temperature and gas conditions help maintain the intended cellular state. Keeping these variables stable supports reproducible studies of cancer cell proliferation, signaling, migration, and responses to treatment.
Aseptic technique helps preserve the controlled culture environment during cell handling and maintenance. When combined with defined media and consistent physical conditions, it reduces variation introduced during routine work and supports reliable comparisons between experiments. This consistency is particularly important when researchers evaluate cancer-cell behavior or compare responses to different therapeutic strategies.
Cells should be passaged or harvested at suitable growth stages rather than at arbitrary times. The selected stage can influence the consistency of material used for downstream experiments, including analyses of proliferation, migration, signaling, and drug response. Standardizing these decisions across experiments improves comparability and helps researchers interpret treatment-related differences more confidently.
Regular monitoring helps confirm that cells remain within the intended culture conditions and that their growth is progressing appropriately. It also provides the basis for deciding when to continue maintenance, passage the cells, or harvest them. In cancer research, this timing supports consistent material for studying tumor biology and treatment responses.
A typical workflow combines aseptic handling, placement in defined culture media, maintenance under appropriate temperature and gas conditions, and regular observation of growth. Once cells reach a suitable stage, researchers either passage them for continued culture or harvest them for analysis. Applying the same sequence across experiments strengthens reproducibility and experimental comparability.
These protocols support work with tumor-derived cell lines and primary cancer cells, allowing researchers to examine proliferation, migration, signaling, drug responses, and treatment resistance. They also help generate models for investigating cancer biology, evaluating therapeutic strategies, and developing clinically relevant systems. The choice of cultured material can therefore align the experiment with its research objective.