Gonadal Fat Removal
Gonadal Fat Removal - Related Videos
10:43
Dissection of Larval Zebrafish Gonadal Tissue
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Cited by 6 •
2017
Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance.
02:59
C. elegans Gonad Extrusion: A Rapid Dissection Technique
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2023
This video describes a method to dissect gonads from adult C. elegans hermaphrodites, resulting in tissues that are suitable for immunostaining and fluorescent imaging.
03:59
Gonad Microinjection: A Method of Compound Delivery Directly into the Germline of C. elegans
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2023
This video describes microinjection, a common method of creating transgenic C. elegans strains. In the example, we will see microinjection used to introduce a ribonucleoprotein (RNP) complex for CRISPR-bases gene editing.
06:30
An ex vivo Mouse Model for High-Fat Diet-Like Adipose Tissue Inflammation
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2026
Using an ex vivo explant model, small pieces of the rostral portion of visceral white adipose tissue from lean mice are cultured for 7–10 days, enabling the induction and study of adipose tissue inflammation as it occurs during high-fat diet feeding.
01:24
Gonadal and Placental Hormones
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2026
The gonads, namely the testes in males and the ovaries in females, are pivotal in producing gonadal hormones that orchestrate the intricate processes of sexual development and reproduction. In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair growth — and...
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The approach creates a comparison in which a defined adipose depot is absent while other experimental conditions remain unchanged. Researchers can then determine whether changes in tumor behavior are associated with the tissue’s removal rather than simply with obesity or the presence of fat elsewhere. This strengthens cause-and-effect analysis of adipose tissue contributions to cancer.
Removing the depot can alter exposure to hormones, lipids, cytokines, and immune-regulating signals produced or released by adipose tissue. These changes may influence nearby cells as well as distant tissues, allowing investigators to examine how metabolic, inflammatory, and immune communication connects fat depots with tumor biology and the broader disease environment.
The tumor microenvironment includes surrounding cells and signals that can modify tumor behavior. Eliminating a defined fat depot helps researchers test whether adipose-derived communication contributes to that environment, rather than treating fat as only a marker of obesity. Resulting changes in tumor growth, inflammation, metabolism, or treatment response can identify specific relationships for further investigation.
The workflow centers on surgically excising the adipose tissue surrounding the reproductive organs and then comparing outcomes with an appropriate experimental condition in which that depot is retained. Researchers maintain other study conditions as consistently as possible, enabling assessment of how the tissue’s absence affects tumor-related measurements without changing the broader experimental design.
Studies may evaluate tumor growth, metabolic changes, inflammatory responses, immune-related effects, and responses to treatment. Considering several outcomes is important because gonadal adipose tissue can influence cancer through multiple signaling routes. Together, these measurements can show whether the depot affects disease progression directly, modifies the tumor environment, or changes therapeutic effectiveness.
This approach is particularly relevant to studies of obesity-associated cancer, where adipose tissue may help connect altered metabolism and inflammation with tumor behavior. It can also clarify interactions between fat tissue, the tumor microenvironment, and therapy outcomes. By isolating one defined depot, researchers can investigate whether its contribution differs from the broader effects of obesity.