Bivariant System

A bivariant system is a thermodynamic system with two degrees of freedom, meaning that two independent intensive variables can change while the same phases remain in equilibrium. In chemical phase analysis, this behavior follows the Gibbs phase rule, F = C − P + 2, where F is variance, C is the number of components, and P is the number of phases; a one-component, single-phase system is therefore bivariant. Temperature and pressure can commonly vary independently in such a region, while composition variables may apply in multicomponent systems. Identifying bivariant regions helps interpret phase diagrams and predict how heating, compression, or composition changes affect equilibrium.

Bivariant System - Related Videos

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JoVE Journal - Developmental Biology

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Cited by 2 •

2017

Quantification of donor-derived cells is required to monitor engraftment after stem cell transplantation in patients with hemoglobinopathies. A combination of flow cytometry-based cell sorting, colony formation assay, and subsequent analysis of short tandem repeats may be used to assess the proliferation and differentiation of progenitors in the erythroid compartment.

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2026

Coronary endarterectomy tissue samples were processed to isolate single nuclei using an optimized dissociation protocol. The integrity of the isolated nuclei was validated through three complementary approaches: trypan blue exclusion staining to assess membrane integrity, DAPI-based confocal microscopy to confirm nuclear morphology, and flow cytometry to quantify nuclear yield and purity.

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JoVE Journal - Bioengineering
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2026

Instructions are provided for setting up and using a 3D perfusion hollow fiber bioreactor for extracellular vesicle production from MSC and other cell types. This protocol describes a method for producing extracellular vesicles at high concentrations on a continuous basis while controlling cell density in hollow fiber bioreactors.

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

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Cited by 15 •

2012

Lentiviral expression vectors are the most effective vehicles for stably expressing different effector molecules or reporter constructs in dividing and non-dividing mammalian cells and whole organisms. Here we provide a protocol on how to package lentivector expression constructs in pseudoviral particles and to transduce target cells using the pseudoviral particles.

In Situ Surface Temperature Measurement in a Conveyor Belt Furnace via Inline Infrared Thermography

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Cited by 3 •

2020

This protocol describes how to install an infrared camera into a conveyor belt furnace, conduct a customer correction of a factory calibrated IR camera, and evaluate the spatial surface temperature distribution of an object of interest. The example objects are industrial silicon solar cells.

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