Nanoparticle Assembly
Nanoparticle Assembly - Related Videos
06:03
Purification of Self-Assembling Protein Nanoparticles using Affinity Chromatography
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2025
This video showcases the purification of histidine-tagged self-assembled protein nanoparticles or SAPNs using affinity liquid chromatography. The resulting purified SAPN fractions are suitable for vaccine development, holding promise for potential immunotherapeutic applications.
08:09
A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
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2014
A simple, robust and scalable technique to functionalize and self-assemble macroscopic nanoparticle-ligand monolayer films onto template-free substrates is described in this protocol.
08:39
Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
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Cited by 7 •
2017
Here we introduce experimental protocols for the real-time observation of a self-assembly process using liquid-cell transmission electron microscopy.
14:18
Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
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Cited by 1 •
2011
Alkanethiolate stabilized gold colloids known as monolayer protected clusters (MPCs) are synthesized, characterized, and assembled into thin films as an adsorption interface for protein monolayer electrochemistry of simple redox protein like Pseudomonas aeruginosa azurin (AZ) and cytochrome c (cyt c).
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
FAQs
Ligands, molecules attached to nanoparticle surfaces, can promote or limit association between particles. Their interactions help determine whether particles remain dispersed or organize into ordered structures. Because ligand behavior is chemical, changing the surface environment can alter particle spacing and arrangement, allowing researchers to connect molecular interactions with the design of larger functional materials.
These conditions influence the balance among attractive and repulsive interactions that bring nanoparticles together. Concentration affects how frequently particles encounter one another, while temperature, pH, and ionic strength can change ligand interactions, electrostatic forces, solvent effects, or hydrogen bonding. Controlling these variables improves the ability to obtain reproducible structures with targeted organization.
Electrostatic forces can either support association or help keep nanoparticles separated, depending on the chemical environment. Solvent effects also modify how particles and their surface ligands interact. Together with van der Waals attraction and hydrogen bonding, these influences determine whether assembly proceeds toward ordered arrangements, making chemical conditions central to controlling nanoscale structure.
Assembly conditions influence how individual nanoscale building blocks pack and interact, which can change the resulting particle spacing, overall organization, and structural form. These changes are important because assembled structures can display collective optical, electronic, magnetic, or catalytic properties that differ from those associated with isolated building blocks. Structure therefore becomes a design variable in materials chemistry.
A typical workflow begins with nanoscale building blocks and establishes chemical conditions that encourage the desired association. Researchers adjust concentration, temperature, pH, ionic strength, and the surrounding solvent while considering ligand interactions and other intermolecular forces. Coordinating these variables helps guide organization and supports more reproducible formation of ordered nanostructures.
Controlled assembly supports the development of plasmonic materials, chemical sensors, catalysts, and functional coatings. In each case, the arrangement of nanoparticles helps connect nanoscale structure with a useful collective response. Chemical control over spacing, shape, and organization can therefore guide the design of materials whose optical, electronic, magnetic, or catalytic behavior serves a specific application.
Nanoparticle assembly provides a link between molecular chemistry and materials design. Chemical interactions at particle surfaces determine how building blocks organize, while the resulting structures influence measurable material behavior. This structure-property connection gives chemists a way to design functional nanomaterials systematically rather than treating particle organization and performance as unrelated features.