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Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
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Bioengineering
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JoVE Journal Bioengineering
Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

DOI:
10.3791/3441-v

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14:18 min

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October 04, 2011

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, , , ,
1Department of Chemistry, Gottwald Center for the Sciences,University of Richmond, 2Department of Biochemistry and Molecular Biology, Gottwald Center for the Sciences,University of Richmond

Chapters

  • 00:05Title
  • 02:05Synthesis of Hexanethiolate Monolayer Protected Gold Clusters
  • 04:42Dithiol-linked MPC Film Assembly for Protein Monolayer Electrochemistry
  • 08:53Dithiol-linked MPC Film Assembly for Optical Trapping
  • 11:46Representative Results
  • 13:28Conclusion

Summary

Automatic Translation

Automatic Translation

Alkanethiolate stabilized gold colloids known as monolayer protected clusters (MPCs) are synthesized, characterized, and assembled into thin films as an adsorption interface for protein monolayer electrochemistry of simple redox protein like Pseudomonas aeruginosa azurin (AZ) and cytochrome c (cyt c).

Tags

Monolayer Protected Gold NanoparticlesAlkanethiolate LigandsFilm AssembliesProtein Monolayer ElectrochemistryAdsorption PlatformsRedox ProteinsElectron Transfer ReactionsGold Nanoparticle Film AssembliesSelf-assembled MonolayersBrust ReactionUV-Vis SpectroscopyTransmission Electron MicroscopyProton NMRDip Cycle MethodDithiol Linking MoleculesGold Electrode InterfacesFilm Growth

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