Oxygen 17
Oxygen 17 - Related Videos
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 28 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
12:38
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
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2008
This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.
09:08
Detecting Reactive Oxygen Species
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2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
11:56
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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Cited by 1 •
2010
Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.
09:38
Dissolved Oxygen in Surface Water
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Dissolved oxygen (DO) measurements calculate the amount of gaseous oxygen dissolved in surface water, which is important to all oxygen-breathing life in river ecosystems, including fish species preferred for human consumption (e.g. bluegill and bass), as well as decomposer species critical to the recycling of biogeochemical materials in the system. The oxygen dissolved in lakes, rivers, and oceans is crucial for the...
FAQs
Oxygen-17's nuclear spin creates the key analytical advantage: it makes this isotope detectable by nuclear magnetic resonance, or NMR, spectroscopy. The NMR response provides a way to examine oxygen-containing chemical environments rather than relying only on isotope abundance. In chemistry, that capability supports structural characterization and makes oxygen-17 useful for following changes during reactions.
An oxygen-17 label serves as a tracer for the oxygen atoms participating in a chemical process. By comparing where the label appears before and after a reaction or exchange process, chemists can determine whether oxygen atoms have been retained or redistributed. This makes labeling especially useful for testing proposed reaction mechanisms and identifying oxygen-transfer pathways.
Low natural abundance is an important experimental constraint because fewer oxygen-17 nuclei are available for direct observation. Isotopic enrichment increases the amount of the isotope present, improving the practical basis for oxygen-17 NMR measurements and labeling studies. Consequently, researchers must consider enrichment when designing experiments that depend on detecting oxygen exchange or resolving oxygen-related structural information.
A combined workflow introduces the isotope through labeling or enrichment and then examines the resulting sample with NMR spectroscopy. The measurement can characterize molecular structure or follow oxygen exchange. This approach connects experimental design, the observed nuclear signal, and the chemical question of how oxygen is organized or transferred within a molecular system.
An oxygen-17 label can help researchers follow oxygen as water moves through a system. When combined with NMR or isotope analysis, it provides a way to connect observed oxygen signals with movement-related questions. This application extends the method beyond molecular structure, making it useful for investigating water behavior in chemistry and environmental studies.
Oxygen-17 approaches connect molecular-scale measurements with broader studies of mineral formation, atmospheric processes, and biological chemistry. In each area, labeling or isotope-sensitive analysis can provide information about oxygen exchange or distribution within the system being studied. These applications show how chemical measurements can contribute to Earth science, environmental research, and investigations of biological processes.