Water Insoluble
Water Insoluble - Related Videos
11:29
Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
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Cited by 7 •
2012
A new species of cellular prion protein (PrPC) has recently been identified in uninfected human brains using the methods described here. These methods can be used to isolate various PrP species, while some of them are also useful in isolating other misfolded protein aggregates from human brains.
09:35
Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain
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Cited by 45 •
2017
An abbreviated fractionation protocol for the enrichment of detergent-insoluble protein aggregates from human postmortem brain is described.
05:36
Isolation of Detergent-Insoluble Protein Aggregates From Human Postmortem Brain Tissue
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2025
This video explains the process of isolating and enriching detergent-insoluble protein aggregates from frozen postmortem human brain tissue. It demonstrates key steps, including homogenization, detergent treatment, sonication, and ultracentrifugation to separate and purify the insoluble aggregates. The final step involves solubilizing the aggregates in urea buffer for further analysis.
09:27
Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
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Cited by 46 •
2016
Here, we present a protocol for the isolation of increasingly insoluble/aggregated alpha-synuclein (α-syn) from post-mortem human brain tissue. Through the utilization of buffers with increasing detergent strength and high-speed ultracentrifugation techniques, the variable properties of α-syn aggregation in diseased and non-diseased tissue can be examined.
08:38
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
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Cited by 6 •
2011
Testing protein-protein interaction is indispensable for dissection of protein functionality. Here, we introduce an in vitro protein-protein binding assay to probe a membrane-immobilized protein with a soluble protein. This assay provides a reliable method to test interaction between an insoluble protein and a protein in solution.
FAQs
Dissolution depends on the balance between the energy needed to separate particles in the compound and the interactions formed between those particles and water. If the energy cost of disrupting the substance exceeds the stabilization provided by water, only limited dissolution occurs. This balance explains why both molecular interactions and crystal structure influence observed solubility.
Nonpolar molecules generally cannot establish favorable hydrogen-bonding or dipole interactions with water. As a result, water provides less stabilization for separated molecules than it does for substances with compatible interactions. This behavior is distinct from the effect of crystal lattice strength, which can restrict dissolution even when a compound has some capacity to interact with water.
Solubility can shift when temperature changes, when pH alters the chemical environment, or when the compound is chemically modified. These adjustments change the balance between the substance and water, sometimes making dissolution more or less favorable. Controlling such conditions helps chemists tune separation behavior, precipitation, and the delivery of chemical species.
In liquid-liquid extraction, limited water solubility helps keep a compound preferentially associated with another liquid phase rather than the aqueous phase. The researcher selects a solvent system that supports this separation, then allows the phases to divide before collecting the desired layer. The approach is useful for separating chemical species according to their differing solvent preferences.
A compound can be brought out of solution when conditions reduce its solubility, producing solid material that can be separated from the remaining liquid. Filtration then retains the precipitated particles while the liquid passes through. This sequence provides a practical way to isolate a substance and is especially useful when controlled solubility changes are available.
Solvent selection determines whether a compound can be dispersed, transferred, or processed effectively despite its limited interaction with water. Chemists consider the substance’s molecular interactions and the intended separation or delivery step when choosing conditions. This principle supports formulation design by helping control how chemical species are incorporated, maintained, or released in a system.