X-ray Luminescence Imaging
X-ray Luminescence Imaging - Related Videos
07:54
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
0 Views •
Cited by 14 •
2015
Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.
08:45
Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring
0 Views •
Cited by 8 •
2012
Use of Cerenkov Luminescence Imaging (CLI) for monitoring preclinical cancer treatment is described here. This method takes advantage of Cerenkov Radiation (CR) and optical imaging (OI) to visualize radiolabeled probes and thus provides an alternative to PET in preclinical therapeutic monitoring and drug screening.
10:39
Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
0 Views •
Cited by 10 •
2016
The measurement protocol and data analysis procedure are given for obtaining transverse coherence of a synchrotron radiation X-ray source along four directions simultaneously using a single 2-D checkerboard phase grating. This simple technique can be applied for complete transverse coherence characterization of X-ray sources and X-ray optics.
07:48
High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
0 Views •
Cited by 2 •
2022
Here, we present a protocol for high-resolution optical detection of chemical information around implanted medical devices with X-ray excited luminescence chemical imaging (XELCI). This novel imaging technique is developed in our lab which enables studying implant-associated infection biochemistry.
01:24
X-ray Imaging
0 Views •
2023
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
FAQs
Chemical composition determines how a material absorbs X-rays and how the deposited energy is redistributed within its electronic structure. These pathways govern whether the energy produces visible or near-infrared emission and influence the measured signal. Comparing materials with different compositions therefore helps connect chemical or materials design with observed luminescence behavior.
Luminescent probes and scintillators provide the emission response that makes absorbed X-ray energy detectable. Their chemical composition and electronic structure affect the conversion of X-ray energy into photons, while their placement determines where signals appear in the image. This makes them central to designing X-ray-responsive systems and interpreting their spatial distributions.
The technique records emitted photons as an image rather than treating the entire sample as a single undifferentiated signal. Spatial differences in luminescent response can therefore reveal where an X-ray-responsive material is located and how it is distributed. This spatial mapping is particularly useful when chemically distinct materials occur within complex samples.
A basic workflow exposes a luminescent material or sample to X-rays, allows the resulting visible or near-infrared photons to be generated, and records those photons with a detector. The recorded image is then used to examine the material’s location and distribution. In chemistry, the measured pattern can be related to composition and emission behavior.
Researchers can use the method to examine how phosphors and nanomaterials respond after absorbing X-rays and to map where those materials occur in a sample. Because the response depends on composition and electronic structure, imaging results can support comparisons among candidate materials. This links spatially observed emission with the design and characterization of X-ray-responsive systems.
X-ray Luminescence Imaging can provide spatial information about luminescent materials while also supporting analysis of their emission behavior. Applications include analytical imaging, materials characterization, and studies of coordination compounds and other X-ray-responsive systems. In biomedical research, mapping signals in complex samples offers an additional context for evaluating where these materials are distributed.