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Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
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JoVE Journal Chemistry
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

DOI:
10.3791/52370-v

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07:54 min

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March 12, 2015

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,
1X-ray Science Division, Advanced Photon Source,Argonne National Laboratory, 2Department of Physics and Astronomy,Northwestern University

Chapters

  • 00:05Title
  • 01:06Preparing Plates with Affixed Sterile Windows
  • 02:38Fixing the Cells
  • 04:48Sample Mounting and Imaging
  • 06:33Images after Successful and Unsuccessful Sample Processing
  • 07:14Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.

Tags

Keywords: X-ray Fluorescence ImagingChemical FixationAdherent CellsMetal DistributionCryo-fixationAldehyde FixationChemical BiologyMaterials ScienceGeoscienceCultural Heritage

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