Acid-fast Culture
Acid-fast Culture - Related Videos
01:26
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
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2024
Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture. Gram Stain The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
07:56
Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol
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Cited by 17 •
2018
The AWESAM protocol described here is optimal for culturing murine astrocytes in isolation from other brain cells in a fast, simple, and inexpensive manner. AWESAM astrocytes exhibit spontaneous Ca2+ signaling, morphology, and gene expression profiles similar to astrocytes in vivo.
09:41
Establishment of an Extracellular Acidic pH Culture System
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Cited by 13 •
2017
The acidic tumor microenvironment plays critical roles in tumor progression. To assess the effects of acidic extracellular pH on cancer cells in vitro, we established simple acidic culture systems.
04:02
A Hyaluronic Acid-Based Hydrogel for 3-Dimensional Culture of Glioblastoma Cells
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2025
This video demonstrates how gliomaspheres, derived from brain tumors, are separated into single cells and encapsulated in a hyaluronic acid-based hydrogel. This process promotes cell-cell interactions, forming a structured, three-dimensional glioblastoma model.
07:13
Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
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Cited by 19 •
2014
Treatment of primary mouse osteoblasts with retinoic acid produces a homogeneous population of ramified cells bearing morphological and molecular features of osteocytes. The method overcomes the difficulty of obtaining and maintaining primary osteocytes in culture, and can be advantageous to study cells derived from transgenic...
FAQs
Mycobacterial cell walls contain abundant lipids, including mycolic acids, that help retain the primary stain after acid-alcohol treatment. This acid-fast behavior provides a laboratory clue when colonies or signals appear in culture. It helps distinguish organisms with this staining property, although culture also supplies viable material for later identification and susceptibility testing.
Solid media and liquid culture systems provide different ways to support the growth of slow-growing mycobacteria. On solid media, growth may be recognized through colonies, whereas liquid systems can be interpreted through culture signals. Using either format allows the laboratory to recover viable organisms from suitable clinical specimens for additional testing.
Clinical specimens may contain contaminating organisms or material that interferes with recovery, so laboratories process them before inoculation. Processing can reduce contamination and, when appropriate, concentrate the specimen. These steps improve the suitability of the material placed into culture systems and support more reliable recovery of slow-growing mycobacteria.
A stain can indicate acid-fast organisms based on their retention of primary stain after acid-alcohol treatment, but culture addresses whether viable organisms can be recovered and grown. That distinction matters clinically because recovered organisms provide material for species identification and antimicrobial susceptibility testing, which can guide management and help evaluate drug-resistant disease.
The laboratory first processes the clinical specimen to reduce contamination and may concentrate it when appropriate. The prepared material is then inoculated into solid media, liquid culture systems, or both, depending on the laboratory workflow. Growth is assessed through colonies or culture signals, followed by recognition of acid-fast organisms and further testing.
Culture is particularly valuable when clinicians need confirmation of tuberculosis or another mycobacterial infection and when viable organisms are needed for follow-up testing. The recovered material can support species identification and antimicrobial susceptibility testing. Results therefore contribute not only to patient management, but also to infection control and surveillance of drug-resistant disease.