Kidney Biopsy
Kidney Biopsy - Related Videos
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
01:45
Kidney Structure
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2019
The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries. The renal cortex is the thick outer layer of the kidney. It...
10:42
Normothermic Machine Perfusion of Rat Kidneys for Transplantation
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2026
This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.
03:14
Vibratome Sectioning of Whole Murine Kidney: A Technique to Generate Thin Sections of Murine Kidney
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2023
In this video, we demonstrate the vibratome sectioning of a whole mouse kidney to obtain thin slices. These sliced tissue sections can be stored at low temperatures until further downstream analysis.
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
FAQs
Each examination method contributes a different view of renal tissue. Light microscopy evaluates overall tissue architecture, while immunofluorescence and electron microscopy provide additional information about tissue features that may not be evident with routine visualization alone. Combining these approaches gives clinicians a more complete basis for identifying disease patterns and assessing their severity.
The tissue findings allow clinicians to compare abnormalities across glomeruli, tubules, interstitium, and blood vessels. This structural information helps separate conditions that can produce similar clinical problems, including glomerulonephritis and nephrotic syndromes. Distinguishing among these patterns is important because the diagnosis, expected course, and treatment approach may differ.
Interpretation considers several renal compartments rather than a single tissue component. Glomeruli, tubules, interstitium, and blood vessels are examined for disease-related changes using the available microscopy methods. Reviewing these structures together helps characterize both the location and extent of injury, supporting a more specific clinical assessment than symptoms or laboratory findings alone.
In transplant care, tissue examination provides objective evidence that can help distinguish rejection from other causes of graft dysfunction. The microscopic assessment of renal structures, supported by immunofluorescence and electron microscopy when relevant, gives clinicians direct pathological information. This evidence can support treatment decisions and help monitor the transplant over time.
The procedure generally begins with image guidance to identify the sampling site, followed by passage of a specialized needle through the skin to remove a small renal tissue sample. The specimen is then examined by light microscopy, immunofluorescence, and electron microscopy. These sequential assessments connect tissue findings with the patient’s clinical question.
Clinicians use the findings when they need to identify the cause and severity of kidney disease, distinguish among clinically important conditions, or evaluate a transplanted kidney. The results can guide selection of treatment, contribute to prognosis estimates, and provide an objective reference for assessing disease progression or response to therapy.