Fetal Vessel On-chip
Fetal Vessel On-chip - Related Videos
09:12
In Vitro Model of Fetal Human Vessel On-chip to Study Developmental Mechanobiology
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Cited by 1 •
2023
Described here is a simple workflow to differentiate endothelial cells from human pluripotent stem cells followed by a detailed protocol for their mechanical stimulation. This allows for the study of the developmental mechanobiology of endothelial cells. This approach is compatible with downstream assays of live cells collected from the culture chip after mechanical stimulation.
12:17
The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo
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Cited by 19 •
2017
We present a detailed method to study human placental physiology in vivo at term. The method combines blood sampling from the incoming and outgoing vessels on the maternal and fetal sides of the placenta with ultrasound measurements of volume blood flow and placental tissue sampling.
14:19
Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction
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Cited by 11 •
2013
We describe examination of fetal cardiac function with contemporary functional fetal echocardiography and fetoplacental Doppler ultrasound using the VisualSonics VEVO 2100 microultrasound in a surgically induced model of intrauterine fetal growth restriction in a rabbit.
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
08:04
Murine Fetal Echocardiography
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Cited by 7 •
2013
Fetal and perinatal death is a common feature when studying genetic alterations affecting cardiac development. High-frequency ultrasound imaging has improved 2-D resolution and can provide excellent information on early cardiac development and is an ideal method to detect the impact on cardiac structure and function prior to death.
FAQs
Fluid flow through perfused channels generates shear stress at the endothelial cell surface. These mechanical forces can influence vessel formation and remodeling, while the controlled channel environment lets investigators examine responses outside the embryo. This makes flow a tunable experimental variable for studying how physical conditions shape developing vasculature.
Developing vessels respond to more than fluid forces. Cellular interactions and molecular signals work alongside mechanical cues to shape vascular development. A fetal vessel on-chip provides a controlled setting for examining these influences together, helping researchers separate interacting developmental factors that are difficult to investigate directly within living embryonic or placental tissues.
The model can support investigation of vessel remodeling and barrier function in addition to vessel formation. Remodeling addresses how vascular structures change over time, whereas barrier studies focus on how effectively the vessel interface functions. Considering these processes together helps connect structural development with the functional properties of fetal vasculature.
A typical setup combines endothelial cells with small microfluidic channels that can be perfused under controlled conditions. The channel geometry supplies a defined environment, and fluid movement introduces shear stress. Together, these components allow investigators to study developing vessel behavior while controlling the laboratory context more precisely than in many tissue-based observations.
In developmental biology, the platform helps researchers examine how mechanical forces, cellular interactions, and molecular signals shape fetal vasculature. It is especially useful when direct investigation in embryos or placental tissue is difficult. By recreating selected developmental features in the laboratory, the system supports focused studies of vascular formation and function.
Fetal vessel on-chip systems can support studies of placental and embryonic vascular disorders, as well as responses to drugs. Researchers can observe how developing vessel structures and barrier-related functions respond under controlled conditions. This provides a laboratory approach for investigating developmental mechanisms and treatment effects without relying exclusively on direct studies in living tissues.