Lr-white Embedding
Lr-white Embedding - Related Videos
08:36
A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology
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Cited by 21 •
2016
Here we present methodology for the production of a focal stroke in murine white matter by local injection of an irreversible endothelial nitric oxide synthase (eNOS) inhibitor (L-Nio). Presented are two stereotactic variations, retrograde neuronal tracing, and fresh tissue labeling and dissection that expand the potential applications of this technique.
09:04
Yeast Colony Embedding Method
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Cited by 7 •
2011
A method for embedding yeast colonies allowing sectioning for light and electron microscopy. This protocol allows determination of the distribution of sporulated cells and pseudohyphal cells within colonies providing a new tool toward understanding the organization of cell types within a fungal community.
03:08
Embedding Adult Drosophila Heads in Paraffin: Preparing Tissue for Histology
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2023
This video describes how to embed Drosophila heads in paraffin using the collar method, a technique that prepares fly heads for serial sectioning. Paraffin sections are used for histological examinations of the brain's anatomy and for the assessment of neurodegeneration. The featured protocol demonstrates how multiple flies can be processed in one preparation, thereby increasing time-efficiency and minimizing experimental artifacts.
08:00
Decoding Natural Behavior from Neuroethological Embedding
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2025
This protocol gives an integrated framework based on advanced computational neuroethological methods to understand brain coding in naturalistic contexts.
03:28
White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup
0 Views •
2025
In this video, we demonstrate the surgical procedure to isolate the subcutaneous white adipose tissue and interscapular brown adipose tissue from a newborn mouse.
FAQs
The resin must infiltrate the specimen after fixation and dehydration so it can surround internal structures before polymerization. Because LR White has relatively low viscosity, it can support preservation of tissue architecture while forming a solid block suitable for sectioning. Effective infiltration is therefore important for retaining organized cellular relationships during microscopic examination of developing tissues.
These stages prepare the specimen for stable microscopic analysis. Fixation preserves biological structure, dehydration prepares the tissue for resin infiltration, and polymerization converts the infiltrated resin into a hard supportive block. The resulting block can be trimmed and sectioned, allowing structural features to remain positioned for detailed light or electron microscopic examination.
The technique combines physical support with preservation of selected molecular targets. Once the resin has polymerized, the specimen can be sectioned for examination of tissue architecture and cellular organization. Its compatibility with selected immunolabeling procedures can also help researchers investigate molecular targets alongside morphology, which is useful when studying how cells differentiate and arrange themselves during development.
Preparation proceeds through fixation, dehydration, resin infiltration, and polymerization. After the specimen is encased in the hardened acrylic resin, the block is trimmed to expose the region of interest. Researchers can then produce thin or ultrathin slices for microscopy. This workflow connects preservation of the original specimen with the section quality needed for structural analysis.
Researchers can use this approach when they need to examine embryos, tissues, or cellular structures during growth and differentiation. It is particularly useful for relating tissue architecture to morphogenesis, the formation and shaping of structures, or to developmental defects. Light and electron microscopy provide complementary levels of structural detail from prepared sections.
Sections prepared with this method can reveal tissue architecture, cellular organization, and fine cellular structures. In developmental studies, those observations help investigators compare structural changes during growth and differentiation or examine abnormalities associated with developmental defects. When selected immunolabeling procedures are compatible, microscopy may also connect observed morphology with the distribution of molecular targets.