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Publisher Subscriber - Related Videos
06:17
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
0 Views •
Cited by 6 •
2018
Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.
07:20
Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
0 Views •
Cited by 1 •
2014
This protocol describes the use of amide coupling reactions of isonicotinic acid and diaminoalkanes to form bridging ligands suitable for use in the synthesis of multinuclear platinum complexes, which combine aspects of the anticancer drugs BBR3464 and picoplatin.
05:12
Melting Point
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2023
Source: Vy M. Dong and Jan Riedel, Department of Chemistry, University of California, Irvine, CA One of the most important properties of a crystalline solid is its melting point. It can be used to determine the purity of a known compound and gives important information about the stability of the formed crystals.
05:40
Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper
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2019
A protocol for the non-destructive analysis of the fiber content and relative age of paper.
09:13
An Introduction to Caenorhabditis elegans
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2023
Caenorhabditis elegans is a microscopic, soil-dwelling roundworm that has been powerfully used as a model organism since the early 1970’s. It was initially proposed as a model for developmental biology because of its invariant body plan, ease of genetic manipulation and low cost of maintenance. Since then C. elegans has rapidly grown in popularity and is now utilized in numerous research endeavors, from studying the forces at work during locomotion to studies of neural circuitry. This video...
FAQs
The broker uses the topic or channel named by the publisher to identify the appropriate subscriber group. It then distributes the message to every subscriber registered for that topic rather than requiring the publisher to track individual recipients. This routing step centralizes message distribution and allows consumers to receive information asynchronously.
Decoupling lets producers and consumers change independently because the publisher does not need direct knowledge of the receiving components. Engineering teams can add, remove, or update subscribers with minimal changes to message-producing software. This separation also supports scalable event-driven architectures, where different components respond to information as it becomes available.
Message ordering and duplication are important design concerns because distributed communication can produce results that differ from the intended sequence or deliver the same message more than once. Engineers must account for these conditions when designing a publisher-subscriber system, particularly when subscribers process telemetry, monitoring information, or other event streams.
Direct communication requires a producer to address a specific consumer, creating tighter dependencies between the two components. Publisher-subscriber replaces that direct relationship with topic-based routing through a broker. As a result, multiple consumers can receive the same information, while the producer remains separated from changes in the consuming components.
First, a producer prepares information and assigns it to a named topic or channel. The broker receives that message and identifies subscribers registered for the same topic. It then forwards the message to those subscribers, which consume it independently and asynchronously. This workflow separates message creation from message handling.
The pattern is used in distributed software, telemetry pipelines, industrial monitoring, and Internet of Things applications. These settings generate information that may need to reach multiple components without tightly coupling the source to each destination. Its value is greatest when systems benefit from scalable, event-driven communication among changing or distributed components.