Plant Pathogen Detection

Plant pathogen detection is the identification of disease-causing organisms, including fungi, bacteria, viruses, and nematodes, in plants or environmental samples. It combines symptom assessment with methods such as microscopy, culture, immunoassays, and nucleic acid amplification, which detect characteristic structures, antigens, or genetic sequences from pathogens. Accurate detection supports early disease diagnosis, surveillance, quarantine, and targeted management in agricultural and natural ecosystems. It also helps researchers track pathogen spread, distinguish closely related species, and evaluate environmental conditions that influence infection, providing essential evidence for protecting plant health, biodiversity, and food production.

Plant Pathogen Detection - Related Videos

Research

JoVE Journal - Immunology and Infection
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Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

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Cited by 23 •

2009

A cell death-based assay for PTI in Nicotiana benthamiana plants is described.

Research

JoVE Journal - Biology
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Detection of Histone Modifications in Plant Leaves

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Cited by 22 •

2011

A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological processes.

Research

JoVE EoE - Bacterial Pathogenesis and Host Interactions

Single-Cell Analysis of Gene Expression from Plant-Extracted Pathogens

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2026

Source: Rufián, J. S., et al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022).This video demonstrates a method for analyzing gene expression in plant-extracted pathogenic bacteria at the single-cell level using a fluorescently tagged transcription factor. Confocal imaging reveals heterogeneity in virulence gene expression, highlighting functional diversity within a clonal bacterial population.

Co-Infiltration Assay for Screening Pathogen Effectors that Suppress Plant RNA Silencing

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2026

Source: Shi, J., et al. Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates a co-infiltration assay for identifying pathogen effector proteins that suppress RNA silencing in Nicotiana benthamiana 16c. A fluorescent reporter and a candidate effector gene are delivered into plant leaves via Agrobacterium. Normally, RNA silencing degrades reporter transcripts, reducing fluorescence. Suppressor effectors...

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

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Cited by 9 •

2018

Here, we present a protocol to test plant virulence with the plant pathogen Magnaporthe grisea. This report will contribute to the large-scale screening of the pathotypes of fungi isolates and serve as an excellent starting point for understanding the resistant mechanisms of plants during molecular breeding.

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