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The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
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The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

DOI:
10.3791/57675-v

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07:14 min

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August 04, 2018

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, , , , , ,
1Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education,Beijing University of Agriculture, 2College of Plant Protection, State Key Laboratory of Agrobiotechnology, Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management,China Agricultural University

Chapters

  • 00:04Title
  • 01:26Spray Inoculation with a Suspension of M. grisea Conidia
  • 04:35Wounding Inoculation with Mycelium Cubes
  • 05:24Results: Com-1 and Mycelial Plug Inoculated O. Sativa Leaves Display Infection Defects
  • 06:14Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to test plant virulence with the plant pathogen Magnaporthe grisea. This report will contribute to the large-scale screening of the pathotypes of fungi isolates and serve as an excellent starting point for understanding the resistant mechanisms of plants during molecular breeding.

Tags

Plant InfectionMagnaporthe GriseaSpray InoculationWound InoculationPlant PathogenPlant DiseaseRiceBarleyOatmeal MediumSeedlingFilter PaperCulture DishOTA PlatesPathotype Screening

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