Spray Inoculation
Spray Inoculation - Related Videos
03:30
A Spray Inoculation Technique to Establish Fungal Infection in Rice Plant Leaves
0 Views •
2025
This video demonstrates a technique to establish the pathogenic fungus Magnaporthe grisea infection in rice plant leaves. The fungal conidia suspension is sprayed on sections of rice leaf, and the disease progression is assessed by scoring the formation of necrotic lesions.
07:14
The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
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Cited by 9 •
2018
Here, we present a protocol to test plant virulence with the plant pathogen Magnaporthe grisea. This report will contribute to the large-scale screening of the pathotypes of fungi isolates and serve as an excellent starting point for understanding the resistant mechanisms of plants during molecular breeding.
08:14
Measuring Spray Droplet Size from Agricultural Nozzles Using Laser Diffraction
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Cited by 30 •
2016
We present protocols to be used in the measurement of spray droplet size from agricultural nozzles used in both aerial and ground based agrochemical applications. These methods presented were developed to provide consistent and repeatable droplet size data both inter- and intra-laboratory, when using laser diffraction systems.
05:43
Topical Airway Anesthesia for Awake-endoscopic Intubation Using the Spray-as-you-go Technique with High Oxygen Flow
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Cited by 11 •
2017
The topical anesthetic lidocaine was atomized using a high oxygen flow through the working channel of a flexible intubating endoscope to achieve topical airway anesthesia for awake endotracheal intubation. We prefer this modified spray-as-you-go technique for endoscopic intubation to classical bolus application because of higher patient satisfaction and better compliance.
08:26
Spray-Coated Melanin/PEDOT:PSS Films for Sustainable Organic Electrochemical Transistors
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2025
A basic protocol for fabricating high-transconductance Melanin/PEDOT:PSS organic electrochemical transistors (OECTs) using spray coating is described. The method allows precise control over film deposition, thickness, and morphology, providing reproducible devices for sustainable and scalable bioelectronic applications.
FAQs
A nozzle's droplet size helps determine how the liquid inoculum is deposited across the target. Because deposition affects coverage, it can influence how evenly microorganisms reach surfaces, soil, plant material, or other substrates. Comparing applications with controlled droplet characteristics helps researchers distinguish effects of delivery from effects of environmental conditions on later survival or colonization.
Moisture and surrounding environmental conditions shape what happens after droplets reach the target. They can influence microbial survival and the ability to colonize the treated substrate, making the same inoculum perform differently under different conditions. Recording or controlling these factors is important when interpreting establishment, persistence, or biological-treatment performance in environmental experiments.
Coverage determines how widely microorganisms are distributed over the defined target, so it provides context for interpreting where establishment or colonization occurs. Uneven deposition can make microbial activity appear localized even when environmental conditions are similar. Measuring or standardizing coverage helps separate patterns caused by application from patterns produced by the substrate or surrounding environment.
Spray inoculation can be directed toward surfaces, soil, plant material, or other substrates, depending on the environmental question. Using a defined target allows researchers to examine how microorganisms establish in a particular setting rather than treating the environment as uniform. This flexibility supports studies of microbial interactions and biological treatments across controlled and field conditions.
A controlled experiment should specify the microbial suspension, spray nozzle, target area, droplet characteristics, and intended coverage. Researchers also need to account for moisture and other environmental conditions because these variables affect deposition, survival, and colonization. Keeping application conditions consistent improves comparability among treatments and makes differences in microbial outcomes easier to interpret.
Researchers use the technique to evaluate microbial establishment, interactions between organisms and their surroundings, and the performance of biological treatments. Measurements of subsequent survival, colonization, microbial activity, or persistence can show how environmental conditions shape the result. Applying the same protocol under controlled or field conditions also supports comparisons between experimental settings.