Sulfate-driven Anaerobic Oxidation

Sulfate-driven anaerobic oxidation is a microbial redox process in which reduced compounds are oxidized without oxygen while sulfate serves as the terminal electron acceptor, linking carbon and sulfur cycling in anoxic environments. In sediments, anaerobic microorganisms can couple methane oxidation to sulfate reduction, converting sulfate to sulfide through coordinated metabolic exchange between methane-oxidizing archaea and sulfate-reducing bacteria. This process is especially important at marine sediment interfaces and other oxygen-depleted habitats, where it regulates methane transport, sulfide production, and nutrient transformations. Studying it helps explain greenhouse-gas emissions, sediment chemistry, microbial community interactions, and potential strategies for managing contaminated or reducing environments.

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JoVE EoE - Bacterial Growth and Techniques

Preparing an Anaerobic Sludge Culture under Oxygen-Free Conditions

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2025

Source: Wagner, A. O.,et al., Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions. J. Vis. Exp. (2019)This video demonstrates the preparation and inoculation of anaerobic cultures using oxygen-free sludge under strictly controlled, deoxygenated conditions.

Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis

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Cited by 2 •

2016

Microwave technology enables extremely fast synthesis of iron oxide nanoparticles for atherosclerosis plaque characterization. The use of an aminobisphosphonate in the external side of the nanoparticle provides a fast accumulation in the atherosclerotic area.

Silver Staining of Sulfated Glycosaminoglycans: A Staining Method for Visualization of Sulfated Glycosaminoglycans in Polyacrylamide Gels

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2025

In this video, we demonstrate a procedure for combined Alcian blue and silver staining of sulfated glycosaminoglycan oligosaccharide fragments. This combined staining technique significantly amplifies the bands of glycosaminoglycan polymers of different lengths in polyacrylamide gels.

Bacterial Biosensor Assay for Detecting Bioavailable Mercury Under Anaerobic Conditions

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2025

Source: Stenzler, B. R. et. al., An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium. J. Vis. Exp. (2018)This video demonstrates the use of genetically engineered bacterial biosensors to accurately measure the bioavailable fraction of mercury ions under anaerobic conditions. By varying the concentration of magnesium sulfate, the assay ensures that the biosensor selectively responds to free mercury ions, as indicated by changes in fluorescence intensity.

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

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Cited by 100 •

2013

Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile cultivation.

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