Multiplex Short Tandem Repeat
Multiplex Short Tandem Repeat - Related Videos
09:39
DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
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Cited by 9 •
2011
Leprosy, caused by Mycobacterium leprae, is still endemic in many places. In order to learn about the spread and mode of transmission of leprosy, it is important to determine which strain of M. leprae has infected a patient. Variable numbers of tandem repeats (VNTR) typing is one such method.
03:01
Detection of Fish Pathogens Using Variable Number Tandem Repeat (VNTR) Analysis
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2025
Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This video demonstrates the procedure for separating fluorescently labeled variable number tandem repeat (VNTR) amplicons, derived from a fish-pathogenic bacterium, using capillary electrophoresis to generate size-specific electropherogram profiles for bacterial identification.
10:33
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Cited by 5 •
2019
The Multi-Locus Variable-number tandem-repeat Analysis (MLVA) assay presented here enables inexpensive, robust and portable high-resolution genotyping of the fish-pathogenic bacterium Yersinia ruckeri. Starting from pure cultures, the assay employs multiplex PCR and capillary electrophoresis to produce ten-loci MLVA profiles for downstream applications.
07:09
Tandem Mass Spectrometry
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2023
In tandem mass spectrometry a biomolecule of interest is isolated from a biological sample, and then fragmented into multiple subunits in order to help elucidate its composition and sequence. This is accomplished by having mass spectrometers in series. The first spectrometer ionizes a sample and filter ions of a specific mass to charge ratio. Filtered ions are then fragmented and passed to a second mass spectrometer where the fragments are analyzed. This video introduces the principles of...
14:37
Ambient Method for the Production of an Ionically Gated Carbon Nanotube Common Cathode in Tandem Organic Solar Cells
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Cited by 7 •
2014
A method of fabricating, in ambient conditions, organic photovoltaic tandem devices in a parallel configuration is presented. These devices feature an air-processed, semi-transparent, carbon nanotube common cathode.
FAQs
Locus-specific primer pairs provide the selectivity needed to target several STR regions within one PCR reaction. Each primer pair corresponds to a particular locus, while the multiplex design coordinates these targets rather than processing them in separate reactions. This arrangement supports simultaneous amplification of multiple markers, which helps conserve sample and reduce laboratory effort.
After amplification, fluorescently labeled products are separated according to size by capillary electrophoresis. This separation allows the analysis to resolve products from different STR loci and identify their allele patterns. The resulting profile can then provide evidence for individual distinction or inheritance comparisons within the tested sample.
Because STR markers are highly variable, combining results from many loci increases the discriminatory power of the profile compared with relying on a smaller set of regions. This broader allele pattern makes the analysis more informative for distinguishing individuals while also reducing sample use and processing time.
A typical workflow begins with multiplex PCR containing locus-specific primer pairs. The amplified products receive fluorescent labels, undergo size separation by capillary electrophoresis, and yield allele patterns for interpretation. This sequence links targeted DNA amplification to a profile that can be evaluated for identification, kinship, or inheritance.
In human identification and forensic investigations, multiplex STR profiles provide allele patterns that help distinguish individuals. In paternity and kinship testing, the same patterns support inheritance comparisons. Population genetics uses these profiles to examine allele variation across groups. Together, these applications show how one assay can address identity, relationship, and population-level questions.
In genetics, allele patterns from multiple STR loci can be used to study whether observed profiles are consistent with inheritance relationships. This makes the approach useful for paternity and kinship testing, where the objective is not simply to distinguish people, but to evaluate relatedness through patterns distributed across several variable DNA regions.